Characterization of α-Gal Epitope in Cells and Tissues from Homozygous α-1,3-Galactosyltransferase Knockout Pigs

Hwang, In-Sul; Kwon, Dae-Jin; Kwak, Tae-Uk; Oh, Keon Bong; Ock, Sun-A; Chung, Hak-Jae; Im, Gi-Sun; Hwang, Seongsoo

추천

검색

자료유형: 학술저널

저자정보: Hwang, In-Sul (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) Kwon, Dae-Jin (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) Kwak, Tae-Uk (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) Oh, Keon Bong (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) Ock, Sun-A (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) Chung, Hak-Jae (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) Im, Gi-Sun (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) Hwang, Seongsoo (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration)

저널정보: 한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제39권 제4호

발행연도: 2015.1

수록면: 127 - 132 (6page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

To overcome the hyperacute immune rejection during pig-to-non-human primates xenotranasplantation, we have produced and bred ${\alpha}$-1,3-galactosyltransferase knock-out ($GalT^{-/-}$) pigs. In this study, the somatic cells and tissues from the $GalT^{-/-}$ pigs were characterized by an analysis of the expression of Gal${\alpha}$-1,3-Gal (${\alpha}-Gal$) epitope. Briefly, ear fibroblast cell lines of 19 homozygous $GalT^{-/-}$ pigs were established and cryopreserved. The expression of ${\alpha}-Gal$ epitope in the cells was measured by fluorescence activated cell sorter (FACS) analysis using BS-I-B4 lectin. Also, the homozygous ($GalT^{-/-}$) cells and tissues samples were immunostained with BS-I-B4 lectin for analysis of ${\alpha}-Gal$ epitope expression. The results showed that the expression of ${\alpha}-Gal$ epitope in $GalT^{-/-}$ cells (0.2 %) were significantly (p<0.05) down-regulated to the range of cynomolgus monkey fibroblast (0.2 %) cells compared to heterozygous ($GalT^{-/+}$) (9.3 %) and wild type ($GalT^{+/+}$) (93.7 %) fibroblast cells. In the immunostaining results, while the expression of ${\alpha}-Gal$ epitope was detected a partly in $GalT^{-/+}$ cells and mostly in $GalT^{+/+}$ cells, it was almost not detected in the $GalT^{-/-}$ cells. Also, immunostaining results from various tissues of the $GalT^{-/-}$ pig showed that the expression of ${\alpha}-Gal$ epitope was not detectable, whereas various tissues from $GalT^{+/+}$ pig showed a strong expression of ${\alpha}-Gal$ epitope. Our results demonstrated that ${\alpha}-Gal$ epitope expressions from $GalT^{-/-}$ pigs were successfully knocked out to prevent hyperacute immune rejection for further study of xenotransplantation.

#Xenotransplantation #${alpha}$-1,3-Galactosyltransferase #$GalT^{-/-}$ pigs #${alpha}-Gal$ epitope #BS-I-B4 lectin

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!

이 논문의 저자 정보

Hwang, In-Sul

소속기관 농촌진흥청 국립축산과학원 동물바이오공학과

주요연구분야 농수해양학 > 축산학

논문수 8 이용수 15

권대진

소속기관 전북대학교 국제농업개발협력센터

주요연구분야 농수해양학 > 농학 농수해양학 > 축산학

논문수 26 이용수 68

Kwak, Tae-Uk