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논문 기본 정보

자료유형
학술저널
저자정보
Bae, Hyo-Kyung (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) Kim, Soo-Hyun (College of Animal Life Science, Kangwon National University) Lee, Sung-Young (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) Hwang, In-Sun (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) Park, Choon-Keun (College of Animal Life Science, Kangwon National University) Yang, Boo-Keun (College of Animal Life Science, Kangwon National University) Cheong, Hee-Tae (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
저널정보
한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제37권 제1호
발행연도
2013.1
수록면
51 - 55 (5page)

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The present study was conducted to examine the effect of antioxidant treatment during parthenogenetic activation procedure on the reactive oxygen species (ROS) levels and in vitro development of porcine parthenogenetic embryos. Porcine in vitro matured oocytes were activated by a combination of electric stimulus and 2 mM 6-dimethylaminopurine (6-DAMP) before in vitro culture. During the activation period, oocytes were treated with $50{\mu}M$ ${\beta}$-mercaptoethanol (${\beta}$-ME), $100{\mu}M$ L-ascorbic acid (Vit. C) or $100{\mu}M$ L-glutathione (GSH). To examine the ROS level, porcine parthenogenetic embryos were stained in $10{\mu}M$ dichlorohydrofluorescein diacetate ($H_2DCFDA$) dye 20 h after culture, examined under a fluorescence microscope, and the fluorescence intensity (pixels) were analyzed in each embryo. The parthenogenetic embryos were cultured for 6 days to evaluate the in vitro development. The apoptosis was measured by TUNEL assay. The $H_2O_2$ levels of parthenogenetic embryos were significantly lower in antioxidant treatment groups ($26.9{\pm}1.6{\sim}29.1{\pm}1.3$ pixels/embryo, p<0.05) compared to control ($33.2{\pm}1.7$ pixels/embryo). The development rate to the blastocyst stage was increased in antioxidant treatment groups (32.0~32.5%) compared to control (26.9%, p<0.05), although, there was no difference in apoptosis among groups. The result suggests that antioxidant treatment during parthenogenetic activation procedure can inhibit the ROS generation and enhance the in vitro development of porcine parthenogenetic embryos.

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