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논문 기본 정보

자료유형
학술저널
저자정보
Jeong, Jin Young (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) Kim, Jang Mi (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) Rajesh, Ramanna Valmiki (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) Suresh, Sekar (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) Jang, Gul Won (Division of Planning and Coordination, National Institute of Animal Science, Rural Development Administration) Lee, Kyung-Tai (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) Kim, Tae Hun (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) Park, Mina (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) Jeong, Hak Jae (Division of Animal Biotechnology, Nationa) Kim, Kyung Woon Cho, Yong Min Lee, Hyun-Jeong
저널정보
한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제37권 제4호
발행연도
2013.1
수록면
219 - 224 (6page)

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Muscular satellite cell (SC), which is stem cell of postnatal pig, is an important for study of differentiation into adipogenesis, myogenesis, and osteoblastogenesis. In this study, we isolated and examined from pig muscle tissue to determine capacity in proliferate, differentiate, and expression of various genes. Porcine satellite cells (PSC) were isolated from semimembranosus (SM) muscles of 90~100 days old pigs according to standard conditions. The cell proliferation increased in multi-potent cell by Masson's, oil red O, and Alizarin red staining respectively. We performed the expression levels of differentiation related genes using real-time PCR. We found that the differentiation into adipocyte increased expression levels of both fatty acid binding protein 4 (FABP4) and peroxisome proliferator-activated receptor gamma ($PPAR{\gamma}$) genes (p<0.01). Myocyte increased the expression levels of the myosin heavy chain (MHC), myogenic factor 5 (Myf5), myogenic regulatory factor (MyoD), and Myogenic factor 4 (myogenin) (p<0.01). Osteoblast increased the expression levels of alkaline phosphatase (ALP) (p<0.01). Finally, porcine satellite cells were induced to differentiate towards adipogenic, myogenic, and osteoblastogenic lineages. Our results suggest that muscle satellite cell in porcine may influence cell fate. Understanding the progression of PSC may lead to improved strategies for augmenting meat quality.

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