지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 102 - 113 (12page)
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초록· 키워드
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C-terminal SRC kinase (CSK) is a ubiquitously expressed, cytosolic enzyme that phosphorylates and inactivates several SRC family protein tyrosine kinases. Recent genomewide association studies have implicated CSK in the regulation of blood pressure. The current study aim is to determine the blood pressure association of the genes regulated by CSK down-regulation. The CSK mRNA expression was downregulated in vascular smooth muscle cells using small interfering RNA (siRNA). CSK mRNA levels fell by 90% in cells that were treated with CSK siRNA; the RNA from these cells was examined by microarray using the Illumina HumanRef-8 v3 platform, which comprises 24,526 reference mRNA probes. On treatment with CSK siRNA, 19 genes were downregulated by more than 2-fold and 13 genes were upregulated by more than 2-fold. Three (CANX, SLC30A7, and HMOX1) of them revealed more than 3 fold differential expression. Interestingly, the HMOX1 SNPs were associated with diastolic blood pressure in the 7551 Koreans using Korea Association REsource data, and the result was supported by the other reports that HMOX1 linked to blood vessel maintenance. Among the remaining 29 differentially expressed genes, seven (SSBP1, CDH2, YWHAE, ME2, PFTK1, G3BP2, and TUFT1) revealed association with both systolic and diastolic blood pressures. The CDH2 gene was linked to blood pressures. Conclusively, we identified 32 differentially expressed genes which were regulated by CSK reduction, and two (HOMX1 and CDH2) of them might influence the blood pressure regulation through CSK pathway.
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참고문헌 (32)
참고문헌 신청
Chen, Y.H / 2002 / Microsatellite polymorphism in promoter of heme oxygenase- 1 gene is associated with susceptibility to coronary artery disease in type 2 diabetic patients / Hum. Genet 111:1~8
Cheng, H.C. / 1996 / Purification of bovine thymus cytosolic C-terminal Src kinase (CSK) and demonstration of differential efficiencies of phosphorylation and inactivation of p56lyn and pp60c-src by CSK / Biochemistry 35:11874~11887
Cho, Y.S / 2009 / A large-scale genome-wide association study of Asian populations uncovers genetic factors influencing eight quantitative traits / Nat. Genet 41:527~534
Chow, L.M / 1995 / The Src and Csk families of tyrosine protein kinases in hemopoietic cells / Semin Immunol 7:207~226
Costa, M. / 1999 / Human DNA helicase VIII: a DNA and RNA helicase corresponding to the G3BP protein, an element of the ras transduction pathway / Nucleic Acids Res 27:817~821
Cheng, H.C. / 1996 / Purification of bovine thymus cytosolic C-terminal Src kinase (CSK) and demonstration of differential efficiencies of phosphorylation and inactivation of p56lyn and pp60c-src by CSK / Biochemistry 35:11874~11887
Cho, Y.S / 2009 / A large-scale genome-wide association study of Asian populations uncovers genetic factors influencing eight quantitative traits / Nat. Genet 41:527~534
Chow, L.M / 1995 / The Src and Csk families of tyrosine protein kinases in hemopoietic cells / Semin Immunol 7:207~226
Costa, M. / 1999 / Human DNA helicase VIII: a DNA and RNA helicase corresponding to the G3BP protein, an element of the ras transduction pathway / Nucleic Acids Res 27:817~821
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