지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 63 - 71 (9page)
이용수
이 논문의 연구 히스토리 (5)
2014
- 이 논문의 후속연구가 궁금하신가요?
- 연관 학술논문 또는 학술발표를 통해 보다 발전된 연구결과를 확인하실 수 있습니다.
- 이 논문의 연구 히스토리 확인하기
초록· 키워드
오류제보하기
Objectives : Lonicerae japonicae Flos(LJF) has been reported to exhibit anti-oxidant, anti-inflammatory, anti-viral, anti-rheumatoid properties. However, it is still largely unknown whether LJF inhibits the ultraviolet(UV)B-induced oxidative damage in human HaCaT keratinocytes. Therefore in this paper, we investigated the anti-oxidative capacity and protective effect of LJF against UVB-induced oxidative demage in human HaCaT keratinocytes. Methods : To evaluate the anti-oxidative activity of LJF extracts, we measured total phenolic contents, total flavonoid contents, antioxidant capacity, and superoxide scavenging activity. To give an oxidative stress to HaCaT cells, UVB was irradiated with $200mJ/cm^2$ to HaCaT cells. To detect the protective effect of LJF against UVB, we measured cell viability, DNA fragmentation and reactive oxygen species (ROS) production. In addition, we performed high-performance liquid chromatography (HPLC) analysis to find a major component of LJF. Results : LJF contained phenolic and flavonoid contents, and showed the anti-oxidant and superoxide scavenging activity. The UVB-induced oxidative conditions led to the cell death, DNA fragmentation and reactive oxygen species (ROS) production. However, pretreatment with LJF reduced oxidative conditions, including inhibition of cell death, DNA fragmentation and ROS production. In addition, we found out chlorogenic acid as major component of LJF. Conclusions : These results could suggest that LJF contained anti-oxidative contents and exhibited protective effects against UVB on human HaCaT keratinocytes. And the effective compound of LJF which could show protective activities against UVB is chlorogenic acid. Thus, LJF and chlorogenic acid would be useful for the development of drug or cosmetics treating skin troubles.
목차
등록된 정보가 없습니다.
참고문헌 (41)
참고문헌 신청
Halliwell B / 1985 / The importance of free radicals and catalytic ions in human diseases / Mol Aspects Med 8(2):89~193
Park SN / 2003 / Antioxidative Properties of Baicalein, Component from Scutellaria baicalensis Georgi and Its Application to Cosmetics / 한국공업화학회지 14(5):657~665
박금주 / 2010 / 가시오가피 추출물의 광노화에 의한 주름형성 억제 효과 / 한국식품영양과학회지 39(1):42~46
Azizi E / 1988 / Skin type, hair color, and freckles are predictors of decreased minimal erythema ultraviolet readiation does / J Am Acad Dermatol 19(1 pt 1):32~38
Applegate LA / 1995 / Two genes contribute to different extents to the heme oxygenase enzyme activity measured in cultured human skin fibroblasts and keratinocytes:implications for protection against oxidant stress / Photochem Photobiol 61(3):285~291
Park SN / 2003 / Antioxidative Properties of Baicalein, Component from Scutellaria baicalensis Georgi and Its Application to Cosmetics / 한국공업화학회지 14(5):657~665
박금주 / 2010 / 가시오가피 추출물의 광노화에 의한 주름형성 억제 효과 / 한국식품영양과학회지 39(1):42~46
Azizi E / 1988 / Skin type, hair color, and freckles are predictors of decreased minimal erythema ultraviolet readiation does / J Am Acad Dermatol 19(1 pt 1):32~38
Applegate LA / 1995 / Two genes contribute to different extents to the heme oxygenase enzyme activity measured in cultured human skin fibroblasts and keratinocytes:implications for protection against oxidant stress / Photochem Photobiol 61(3):285~291
이 논문의 저자 정보
최근 본 자료
댓글(0)
0