지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 69 - 74 (6page)
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Objectives : Since hypopigmentation is known to increase the risk of skin cancer, melanogenesis in the skin needs to be regulated. Here, we evaluated the melanogenesis stimulatory effects of a modified Goagium herbal remedy (HR) and HR+ox bile (Bos taurus domesticus) extract (OBE) to address hypopigmentation disorders. Methods : B16F10 melanoma cells were treated with different dosages of HR and HR+OBE for 24 to 48 h after 1 h of 10 nM ${\alpha}$-melanocyte stimulating hormone (${\alpha}$-MSH). After the treatment, cell viability, tyrosinase activity, melanin synthesis and the expression of genes related to melanin synthesis were measured and the regulation of the ${\alpha}$-MSH signalling through cAMP responding element binding protein (CREB) was determined. Results : HR and HR+OBE with the ranges of $15{\sim}100{\mu}g/mL$ did not affect cell viability in melanoma cells. The 1 h treatment of HR+OBE (50 and $100{\mu}g/mL$) potentiated the phosphorylation of CREB by enhancing ${\alpha}$-MSH signaling and its 24 h treatment increased CREB expression. Consistent with CREB potentiation, their treatment for 24 h, the expression of microphthalmia-associated transcription factor (MIFT), tyrosinase, tyrosinase related protein (TRP)-1 and TRP-2 were increased in realtime PCR. Ultimately, the 48 h treatment of HR+OBE (50 and $100{\mu}g/mL$) increased tyrosniase activity and melanin contents in the melanoma cells in comparison to the control. Conclusions : HR+OBE (50 and $100{\mu}g/mL$) increases melanin synthesis in B16F10 melanoma cells via the stimulation of tyrosinase activity and expression of MIFT, tyrosinase, TRP-1 and TRP-2. HR+OBE can be used as the a possible treatment for hypopigmentation of the skin.
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참고문헌 (20)
참고문헌 신청
Yu JS / 2010 / Effect of combination of taurine and azelaic acid on antimelanogenesis in murine melanoma cells / J Biomed Sci 17(1):S45
Kim do Y / 2009 / Quality of life for Korean patients with vitiligo: Skindex-29 and its correlation with clinical profiles / J Dermatol 36(6):317~322
Felsten LM / 2011 / Vitiligo: a comprehensive overview Part II: treatment options and approach to treatment / J Am Acad Dermatol 65(3):493~514
Alikhan A / 2011 / Vitiligo: a comprehensive overview Part I. Introduction, epidemiology, quality of life, diagnosis, differential diagnosis, associations, histopathology, etiology, and work-up / J Am Acad Dermatol 65(3):473~491
Imokawa G / 1982 / Loss of melanogenic properties in tyrosinase induced by glucosylation inhibitors within malignant melanoma cells / Cancer Res 42(5):1994~2002
Kim do Y / 2009 / Quality of life for Korean patients with vitiligo: Skindex-29 and its correlation with clinical profiles / J Dermatol 36(6):317~322
Felsten LM / 2011 / Vitiligo: a comprehensive overview Part II: treatment options and approach to treatment / J Am Acad Dermatol 65(3):493~514
Alikhan A / 2011 / Vitiligo: a comprehensive overview Part I. Introduction, epidemiology, quality of life, diagnosis, differential diagnosis, associations, histopathology, etiology, and work-up / J Am Acad Dermatol 65(3):473~491
Imokawa G / 1982 / Loss of melanogenic properties in tyrosinase induced by glucosylation inhibitors within malignant melanoma cells / Cancer Res 42(5):1994~2002
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