지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Objective: The prevalence of metabolic syndrome and type 2 diabetes is increasing worldwide. Mitochondrial dysfunction is known to be involved in insulin resistance and obesity, researches have been increasing highly. Astragali Radix extract (ARE) or its main components have been shown to perform comparably to insulin by significantly reducing blood glucose levels in animal models however, the influence on mitochondrial dysfunction are not well understood. Methods: ARE (0.2, 0.5 and 1.0 mg/ml) or metformin (2.5 mM) were treated in C2C12 after 6 day-differentiation. The expressions of adenosine monophosphate (AMP)-activated protein kinase (AMPK) and phosphorylation AMPK, peroxisome proliferators-activated receptror ${\gamma}$ coactivator $1{\alpha}$ ($PGC1{\alpha}$), nuclear respiratory factors 1 (NRF1), mitochondrial transcription factor (Tfam) and myosin heavy chain were detected with western blotting or polymerase chain reaction analysis. The morphological changes were also investigated. Results: ARE dose dependently increased phosphorylation of AMPK and respectively activated mRNA expressions of $PGC1{\alpha}$, NRF1 and Tfam which are mitochondrial biogenesis regulators. Furthermore, there were some morphologic differences of differentiated cells between ARE treatment and control. Conclusions: This study suggests that ARE has the potential to increase muscle mitochondrial function by activating AMPK and $PGC1{\alpha}$.
목차
등록된 정보가 없습니다.
참고문헌 (18)
참고문헌 신청
Wallace DC / 2013 / A mitochondrial bioenergetic etiology of disease / J Clin Invest 123(4):1405~1412
Lee HK / 2010 / Mitochondrial dysfunction and metabolic syndrome−looking for environmental factors / Biochimica et Biophysica Acta 1800(3):282~289
Lowell BB / 2005 / Mitochondrial dysfunction and type 2diabetes / Science 307(5708):384~387
Kim JA / 2008 / Role of mitochondrial dysfunction in insulin resistance / Circ Res 102(4):401~414
추효정 / 2006 / 제2형 당뇨병과 미토콘드리아 / Endocrinology and Metabolism 21(4):266~271
Lee HK / 2010 / Mitochondrial dysfunction and metabolic syndrome−looking for environmental factors / Biochimica et Biophysica Acta 1800(3):282~289
Lowell BB / 2005 / Mitochondrial dysfunction and type 2diabetes / Science 307(5708):384~387
Kim JA / 2008 / Role of mitochondrial dysfunction in insulin resistance / Circ Res 102(4):401~414
추효정 / 2006 / 제2형 당뇨병과 미토콘드리아 / Endocrinology and Metabolism 21(4):266~271
이 논문의 저자 정보
최근 본 자료
댓글(0)
0