지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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PURPOSE: In this study, we tried to find out what kind of exercise was more effective in sleep disorder by comparing melatonin in blood after applying low intensity with high intensity exercise to sleep disordered rats induced by experiment. METHODS: We used male Sprague-Dawley rats which were 8weeks old and weighted 300g. They were supplied with water and food without any restriction. We kept the room temperature at $25^{\circ}C$ and controld the length of day and night in 12 hours blocks, respectively. We divided the rats 60 into 2 groups. To one group we applied low intensity exercise, and to the other we applied high intensity exercise for 15minutes per day over a period of 4 weeks. We extracted the blood from abdominal aorta before, after exercise, moved into EDTA tube, performed centrifugation. We decanted the serum $200{\mu}l$ from the blood into microcentrifuge tube by samples and moved into polypropylene culture tubes with micro pipette. We split enzyme solution $50{\mu}l$ into the tubes with melatonin direct kits and make them react at $37^{\circ}C$ for 2 hours. We split assay buffer $50{\mu}l$ into each tube and mixed melatonin tracer $50{\mu}l$ and melatonin antiserum $50{\mu}l$, respectively. After we made them react in room temperature, we decanted the superficial layer with a centrifuge and measured the activity for 1 minute by competitive method with ${\gamma}$-counter equipment. We draw a standard curve through logit-log graph with CPM(counts per minute) and counted the melatonin by B/B0. We conducted independent t-test to examine the homogeneous of melatonin value of before low-intensity and high-intensity exercise. We performed paired t-test to compare before and after low-intensity and high-intensity exercise, respectively. We carried out independent t-test to compare melatonin value after low-intensity and high-intensity exercise. Significance level was .05. RESULTS: The results were as follows; firstly melatonin was more increased in the group who was exposed to high intensity exercise when we compared before to after high and low intensity exercise, respectively. Secondly, high intensity exercise was more effective than low intensity exercise when we compared the two. CONCLUSION: In conclusion, secretion of melatonin which is the material of sleep improvement could be promoted by high intensity exercise. Low intensity exercise acted as a stress rather than improving sleep and had a negative effect on the secretion of melatonin because the melatonin was affected by stress.
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참고문헌 (37)
참고문헌 신청
Alessio HM / 1993 / Exercise-induced oxidative stress / Med Sci Sports Exerc 25(2):218~224
Arendt J / 2000 / Melatonin, circadian rhythms and sleep / N Engl J Med 343(15):1114~1116
Arendt J / 2005 / Melatonin; characteristics, concerns, and prospects / J Biol Rhythms 20(4):291~303
Cajochen C / 1998 / Evening adminstration of melatonin and bright light: interactions on the EEG during sleep and wakefulness / J Sleep Res 7(3):145~157
Clapp-Lilly KL / 2001 / Melatonin acts as anti-oxidant and pro-oxidant in an organotypic slice culture model of Alzheimer’s disease / NeuroReport 12(6):1277~1280
Arendt J / 2000 / Melatonin, circadian rhythms and sleep / N Engl J Med 343(15):1114~1116
Arendt J / 2005 / Melatonin; characteristics, concerns, and prospects / J Biol Rhythms 20(4):291~303
Cajochen C / 1998 / Evening adminstration of melatonin and bright light: interactions on the EEG during sleep and wakefulness / J Sleep Res 7(3):145~157
Clapp-Lilly KL / 2001 / Melatonin acts as anti-oxidant and pro-oxidant in an organotypic slice culture model of Alzheimer’s disease / NeuroReport 12(6):1277~1280
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