지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Objectives: The aim of this study is to investigate anti-imflammatory and protective effect for intestinal epithelial cells with Atractylodes macrocephae (AM), a traditional Korean Herbal medicine and fermented Atractylodes macrocephae (FAM) with Lactobacillus plantarum. Methods: HCT-116 and Raw 264.7 cells were used in this study. Using NO assay, we measured lipopolysaccharide (LPS)-induced anti-inflammatory effect. We measured permeability of intestinal epithelial cells with transepithelial electrical resistance and horseradish peroxide flux assay. Water soluble tetrazolium salt assay was used to see cell proliferation. All the results were presented in mean and standard deviation. We used Student's t-test for analyzing significance of results. Results: In Raw 264.7 cells NO production decreased 22.4% with pre-treatment of AM and FAM, especially with FAM in high concentration. In HCT-116 cells LPS-induced intestinal permeability had a protective effect with both AM and FAM, which was also tend to be proportional to the concentration. Cell viability increased up to 135.52% after treatment of high concentration of FAM in HCT-116, while there was no significant change in Raw 264.7 cells with herb treatments. Conclusions: These results show evidence that AM, especially fermented ones, significantly reduced intestinal membrane permeability. They also had a protective effect as well as an anti-inflammation effect for HCT-116 and Raw 264.7 cells. This suggest that FAM may be a therapeutic agent for Leaky gut syndrome by reducing intestinal permeability.
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참고문헌 (18)
참고문헌 신청
Farhadi A / 2003 / Intestinal barrier: an interface between health and disease / J Gastroenterol Hepatol 18(5):479~497
Marik PE / 1999 / Intestinal mucosal permeability: mechanism and implications for teatment / Cit Care 27(8):1650~1651
Fasano A / 2011 / Leaky gut and autoimmune diseases / Clinic Rev Allerg Immunol 42:71~78
Lieu Z / 2005 / Tight junctions, leaky intestines, and pediatric diseases / Acta Pediatr 94:386~393
Ukena SN / 2007 / Probiotic Escherichnia coli Nissle 1917 inhibits leaky gut by enhancing mucosal integrity / PLoS One 12:e1308
Marik PE / 1999 / Intestinal mucosal permeability: mechanism and implications for teatment / Cit Care 27(8):1650~1651
Fasano A / 2011 / Leaky gut and autoimmune diseases / Clinic Rev Allerg Immunol 42:71~78
Lieu Z / 2005 / Tight junctions, leaky intestines, and pediatric diseases / Acta Pediatr 94:386~393
Ukena SN / 2007 / Probiotic Escherichnia coli Nissle 1917 inhibits leaky gut by enhancing mucosal integrity / PLoS One 12:e1308
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