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논문 기본 정보

자료유형
학술저널
저자정보
최선호 (농촌진홍청 국립축산과학원) 김성재 (농촌진홍청 국립축산과학원) 조상래 (농촌진홍청 국립축산과학원) 최창용 (농촌진홍청 국립축산과학원) 손준규 (농촌진홍청 국립축산과학원) 김종석 (진돗개사업소) 오석일 (진돗개사업소) 박병진 (진돗개사업소) 김상현 (진도개사업소)
저널정보
한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제34권 제3호
발행연도
2010.1
수록면
253 - 256 (4page)

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초록· 키워드

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This study was performed to investigate the characteristics within ages and freezing tolerance of spermatozoa in Jindo Dog. Experimental animals were selected 12 herds within 1~8 year's old and collected semen for 2 times in a week. Collected semen was evaluated whole volume and sperm number with CASA system (SIAS, Medical Supply, Korea). Then seminal plasma were separated and diluted with modified Tris-egg yolk extender and added 4, 6 and 8% glycerol for 4 times to final concentration and equilibrated for 1.5 hrs. Before and after freezing, equilibrated semen were evaluated the survival rates. Total volume of sperm at 1~2 year old group is as $5.2{\times}10^8\;cells/ml$ largest and there were no significance among groups. The motility of 1~2 year old group is highest as 90.9% and there were significance among groups. Abnormal sperm showed similar among groups. The survival rate in terms of pre-freezing and post-freezing were decreased all levels of glycerol and reveled 87.0% to 64.5% in 4%, 87.5% to 51.9% in 6% and 73.4% to 29.7% in 8%, there were significant difference among the groups (p<0.05). These results suggest that the optimal sperm-freezing methods in Jindo Dog are utilized with modified Tris egg-yolk extender with 4% glycerol and were improve the reproductive activity by these methods.

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