지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Water temperature is key factor influencing growth and reproduction of fish and its increase give rise to various physiological changes including gene expression. Heat shock protein (Hsp), one of the molecular chaperones, is highly conserved throughout evolution and its expression is induced by various stressors such as temperature, oxidative, physical and chemical stresses. Here, we isolated partial cDNA clones encoding 70-kDa Hsp (Hsp70) and $\beta$-actin using reverse transcriptase-PCR (RT-PCR) from gut of Rhynchocypris kumgangensis, a Korean indigenous species and cold-water fish, and investigated expression profiles of Hsp70 under an increase of water temperature using $\beta$-actin as an internal control for RT-PCR. Cloned Hsp70 cDNA of R. kumgangensis showed homology to Ctenopharyngodon idella (96%), Hypophthalmichthys molitrix (96%), Danio rerio (93%) and Oncorhynchus mykiss (81%) Hsp70. Cloned $\beta$-actin cDNA of R. kumgangensis showed homology to D. rerio (98%), H. molitrix (97%), C. idella (97%) and O. mykiss (90%) $\beta$-actin. Both mRNA of Hsp70 and $\beta$-actin were expressed in gut, brain, and liver in R. kumgangensis. Futhermore, expression of Hsp70, in brain, was highly augmented by an increase of water temperature. These results suggest that Hsp70 mRNA expression level in brain can be used as a biological molecular marker to represent physiological stress against an increase of water temperature.
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참고문헌 (27)
참고문헌 신청
Arai, A. / 1995 / Cloning and Characterization of cDNAs for 70-kDa Heat-shock Proteins(Hsp70)from Two Fish Species of the Genus Oryzias / Japanese Journal of Genetics 70(3):423~433
Baek, H. M. / 2002 / Habitat Segregation and Prey Selectivity on Cohabitation Fishes, Phoxinus phoxinus and Rhynchocypris kumgangensis / Korean Journal Ichthyol 14(2):121~131
Chatterjee, S. / 2007 / Synergistic Therapeutic Potential of Dexamethasone and L-arginine in Lipopolysaccharide-induced Septic Shock / The Journal of Surgical Research 140(1):99~108
최철영 / 2006 / 담수사육 감성돔, Acanthopagrus schlegeli의 수온 상승에 따른 HSP90, HSP70 mRNA의 발현 및 혈액 생리학적 변화 / 한국양식학회지 19(4):315~322
Cui, H. / 2011 / Cloning, Characterization, and Expression Analysis of Orange-spotted Grouper(Epinephelus coioides)ILF2 Gene(EcILF2) / Fish & Shellfish Immunology 30(1):378~388
Baek, H. M. / 2002 / Habitat Segregation and Prey Selectivity on Cohabitation Fishes, Phoxinus phoxinus and Rhynchocypris kumgangensis / Korean Journal Ichthyol 14(2):121~131
Chatterjee, S. / 2007 / Synergistic Therapeutic Potential of Dexamethasone and L-arginine in Lipopolysaccharide-induced Septic Shock / The Journal of Surgical Research 140(1):99~108
최철영 / 2006 / 담수사육 감성돔, Acanthopagrus schlegeli의 수온 상승에 따른 HSP90, HSP70 mRNA의 발현 및 혈액 생리학적 변화 / 한국양식학회지 19(4):315~322
Cui, H. / 2011 / Cloning, Characterization, and Expression Analysis of Orange-spotted Grouper(Epinephelus coioides)ILF2 Gene(EcILF2) / Fish & Shellfish Immunology 30(1):378~388
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