지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 719 - 725 (7page)
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초록· 키워드
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To apply TMAH-based Py-MS to a field biological detection system for real-time classification of cell-type, reproducible patterns of the TMAH-based Py-MS spectra was known as a critical factor for classification but was seriously disturbed by quantity of cells injected into pyro-tube. This factor is an exterior variable that could not be complemented by improving the performance of the TMAH-based Py-MS instrument. One of idea to solve the knotty problem has been flashed from "Top-down proteomics for identification of intact microoganisms". That is, biomarker peaks are selected from complicate Py-MS spectra for intact microoganisms by tracing out their origins, based on Py-MS spectra for the featured components of different cell-types, in Top-down approach. This idea has been tested in classification of different Gram-type microoganisms. Through the analyses of spectra for the featured components - peptidoglycan and lipoteichoic acid for Gram-positive cells and lipopolysaccharide and lipid A for Gram-negative cells - with comparing to the spectra the corresponding Gram-type cells in the Top-down approach, biomarker peaks were selected to carry out PCA(Principal Component Analysis) in order to see classification of different Gram-types, resulting in significant improvement of their classification. Furthermore, weighting biomarker peaks on intact cell's spectra, based on the data for the featured components of the Gram-types, contributed to elevate classification performance.
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참고문헌 (9)
참고문헌 신청
Ju Hyun Kim / 2010 / Real-time Classification of Unknown Bio-agents based on Pyrolysis Mass Spectormetry / 한국군사과학기술학회 2010년 종합학술대회 :1025~1028
Plamen A. Demirev / 2005 / Top-Down Proteomics for Rapid Identification of Intact Microorganisms / Anal. Chem 77:7455~7461
Holst, O. / 1992 / Chemical Structure of the Core Region of Lipopolysaccharides in Bacterial Endotoxic Lipopolysaccharides / CRC Press :135~170
Otto Holst / 1996 / Biochemistry and Cell Biology of Bacterial Endotoxin / FEMS Immunology and Medical Microbiology 16:83~104
Yuriy A. Knirel / 2005 / Cold Temperture-induced Modification to Composition and Structure of the Lipopolysaccharide of Yesinia Pestis / Carbohydrate research 340:1625~1630
Plamen A. Demirev / 2005 / Top-Down Proteomics for Rapid Identification of Intact Microorganisms / Anal. Chem 77:7455~7461
Holst, O. / 1992 / Chemical Structure of the Core Region of Lipopolysaccharides in Bacterial Endotoxic Lipopolysaccharides / CRC Press :135~170
Otto Holst / 1996 / Biochemistry and Cell Biology of Bacterial Endotoxin / FEMS Immunology and Medical Microbiology 16:83~104
Yuriy A. Knirel / 2005 / Cold Temperture-induced Modification to Composition and Structure of the Lipopolysaccharide of Yesinia Pestis / Carbohydrate research 340:1625~1630
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