지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 129 - 135 (7page)
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초록· 키워드
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We report the formation of liposomes in a simple procedure using a microfluidic hydrodynamic flow focusing method for the application in gene delivery. We fabricated microfluidic device using soft lithography and polydimethylsiloxane (PDMS) molding technique. Lipid-containing stream was surrounded by aqueous stream and liposomes were formed at the lipid-water interface. Size distribution of liposomes and zeta potential of liposome dispersion were investigated under various flow rate ratio (FRR) and processing temperature. Size distributions of liposomes were measured by dynamic light scattering (DLS), and zeta potential was measured to quantify the colloidal stability. Prepared liposomes were used as a vehicle for gene delivery, and the successful expression of delivered gene was observed by fluorescent microscope.
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참고문헌 (14)
참고문헌 신청
Allen, T.M. / 1989 / Liposomes with prolonged circulation times: factors affecting uptake by reticuloendothelial and other tissues / Biochim. Biophys. Acta 981:27~35
Badyakina, A.O. / 2003 / Membrane Phospholipid Composition ofEscherichia coli Affects Secretion of Periplasmic AlkalinePhosphatase into the Medium / Biochemistry 68:752~759
Bangham, A.D. / 1965 / Diffusion of univalent ions across the lamellae of swollen phospholipids / J. Mol. Biol 13:238~252
Capretto, L. / 2011 / Continuous-flow production of polymeric micelles in microreactors: experimental and computational analysis / J. Colloid Interface Sci 357:243~251
Chapman, C.J. / 1991 / Effects of solute concentration on the entrapment of solutesin phospholipid vesicles prepared by freeze-thaw extrusion / Chem. Phys. Lipids 60:201~208
Badyakina, A.O. / 2003 / Membrane Phospholipid Composition ofEscherichia coli Affects Secretion of Periplasmic AlkalinePhosphatase into the Medium / Biochemistry 68:752~759
Bangham, A.D. / 1965 / Diffusion of univalent ions across the lamellae of swollen phospholipids / J. Mol. Biol 13:238~252
Capretto, L. / 2011 / Continuous-flow production of polymeric micelles in microreactors: experimental and computational analysis / J. Colloid Interface Sci 357:243~251
Chapman, C.J. / 1991 / Effects of solute concentration on the entrapment of solutesin phospholipid vesicles prepared by freeze-thaw extrusion / Chem. Phys. Lipids 60:201~208
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