지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
In this study, we investigate anti-allergic and anti-inflammatory effects of Leonurus sibiricus seed (LSS) extract in basophilic leukemia RBL-2H3 cells. To identify anti-allergic actions of LSS, the degranulation was evaluated in IgE and DNP-BSA stimulated RBL-2H3 cells. At the concentration of $100{\mu}g/ml$ of methanol (MeOH) extract and Methylene chloride (MC) and Ethyl acetate (EtOAc) fractions, the degranulation was significantly inhibited 16.7%, 16.7% and 27.9% respectively. And then, to assess anti-inflammatory effects of LSS, IL-4 and IL-13 mRNA level were detected in PMA/ionomycin (PI)-induced RBL-2H3 cells and cell proliferation and IL-4 mRNA level in isolated splenocytes from Balb/c mice. LSS MeOH extract and MC and EtOAc fractions significantly decreased the level of IL-4 and IL-13 mRNA in PI-induced RBL-2H3 cells and showed inhibitory effects on cell proliferation and expression of IL-4 mRNA level in mouse splenocytes. Taken together, these results suggest that LSS has potential anti-allergic and anti-inflammatory effects and EtOAc fraction is the most effective in regulating immune responses.
목차
등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Kindt, T. J. / 2007 / Kuby Immunology / W. H. Freeman Publishers :426
Bradding, P. / 2006 / The role of the mast cell in the pathophysiology of asthma / J. Allergy Clin. Immunol 117:1277
Maggi, E. / 1998 / The TH1/TH2 paradigm in allergy / Immunotechnology 3:233
Schmid-Grendelmeier, P. / 2002 / Eosinophils express functional IL-13 in eosinophilic inflammatory diseases / J. Immunol 169:1021
Zavorotinskaya, T. / 2003 / Treatment of experimental asthma by long-term gene therapy directed against IL-4 and IL-13 / Mol. Ther 7:155
Bradding, P. / 2006 / The role of the mast cell in the pathophysiology of asthma / J. Allergy Clin. Immunol 117:1277
Maggi, E. / 1998 / The TH1/TH2 paradigm in allergy / Immunotechnology 3:233
Schmid-Grendelmeier, P. / 2002 / Eosinophils express functional IL-13 in eosinophilic inflammatory diseases / J. Immunol 169:1021
Zavorotinskaya, T. / 2003 / Treatment of experimental asthma by long-term gene therapy directed against IL-4 and IL-13 / Mol. Ther 7:155
이 논문의 저자 정보
최근 본 자료
댓글(0)
0