지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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We previously reported that the extract of Taraxacum platycarpum (AF-343) had several biological properties such as skin hydration and anti-inflammatory effects, thereby AF-343 be a promising anti-atopic dermatitis agent. However, few studies have been conducted to evaluate its effect on modulation of extracellular matrix proteins in human skin fibroblasts. The purpose of this study was to investigate the expressions of type I collagen, MMP-1, Smad2/3, and TIMP-1 proteins in AF-343-treated human skin fibroblasts. Human skin fibroblasts were treated by various concentrations of AF-343 (0~2 mg/ml). The expressions of type I collagen, matrix metalloproteinase-1 (MMP-1), Smad2/3, and TIMP-1 proteins were analyzed by Western blot analysis. In addition, level of type I collagen mRNA was analyzed by CAT assay. Expression of type I collagen protein was increased in AF-343-treated human skin fibroblasts by dose and time-dependent manners. Consistent with this result, the expressions of phospho-Smad2/3 in skin fibroblasts were increased and MMP-1 expression was decreased by AF-343 treatment. TIMP-1 expression was not significantly changed in AF-343 treated skin fibroblasts. Extract of Taraxacum platycarpum (AF-343)-induced up-regulation of type I collagen expression was through increased expression of phospho-Smad2/3. These results were occurred combined with down-regulation of MMP-1 in skin fibroblasts. Taken together, this study indicated that AF-343 has property of the modulation of ECM in tissue as well as skin hydration and anti-inflammation.
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참고문헌 (16)
참고문헌 신청
Schütz, K. / 2006 / Taraxacum-a review on its phytochemical and pharmacological profile / J. Ethnopharmacol 107:313
Cheong, H. / 1998 / Desacetylmatricarin, an anti-allergic component from Taraxacum platycarpum / Planta. Med 64:577
Bhogal, R. K. / 2005 / Molecular aspects of regulation of collagen gene expression in fibrosis / J. Clin. Immunol 25:592
Verrecchia, F. / 2006 / Transforming growth factor-beta signaling through the Smad proteins: role in systemic sclerosis / Autoimmun. Rev 5:563
Homebeck, W. / 2003 / Down-regulation of tissue inhibitor of matrix metalloprotease-1 (TIMP-1) in aged human skin contributes to matrix degradation and impaired cell growth and survival / Pathol. Biol 51:569
Cheong, H. / 1998 / Desacetylmatricarin, an anti-allergic component from Taraxacum platycarpum / Planta. Med 64:577
Bhogal, R. K. / 2005 / Molecular aspects of regulation of collagen gene expression in fibrosis / J. Clin. Immunol 25:592
Verrecchia, F. / 2006 / Transforming growth factor-beta signaling through the Smad proteins: role in systemic sclerosis / Autoimmun. Rev 5:563
Homebeck, W. / 2003 / Down-regulation of tissue inhibitor of matrix metalloprotease-1 (TIMP-1) in aged human skin contributes to matrix degradation and impaired cell growth and survival / Pathol. Biol 51:569
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