지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
An analytical methodology based on solid-space extraction (SPE) with with Bond Elut Certify cartridge (Varian, 130 mg) has been developed for the qualification and quantitation of strychnine in blood. After the elution layer was evaporated, the residue was reconstituted with methanol for GC/MS. Internal standard was used 10 mg/l dextromethorphan. Strychnine is a potent central nervous stimulant and convulsant, and an alkaloid found in seeds of Strychnos nux-vomica. It was used therapeutically to improve circulation and muscle tone in oral or intramuscular doses of 0.05~8 mg. The fatal dose of strychnine for humans is 50~100 mg. A man was found dead lying curled up the corner of the large room in a roof house after the fire fighter opened a locked door inside to put out the fire. The postmortem blood and gastric contents were analyzed for toxicological testing. Strychnine and brucine were detected using GC/MS first in gastric contents extracts. The contents of strychnine was 0.083 mg/l in heart blood, 0.088 mg/l in peripheral blood and 4.0 mg/kg in gastric contents, respectively. Method validation was carried out in terms of linearity, accuracy, precision (intraday, interday) in blood. The assay is linear over 0.05~10 mg/l ($r^2$=0.999). Limit of detection (LOD) and limit of quantitation (LOQ) in blood were determined 0.02 mg/l (S/N=3) and 0.07 mg/l (S/N=10), respectively. Accuracy (bias%) of strychnine with 0.1, 1 and 10 mg/l was 12.0% (n=6), 9.3% (n=6) and 6.9% (n=6), respectively. Intraday precision (CV%) of strychnine with, 0.1, 1 and 10 mg/l were 6.4%, 10.4%, 1.2% (n=6), respectively. Interday precision (CV%) of strychnine with 0.1, 1 and 10 mg/l over three days were 24.0%, 18.5%, 13.8% (n=18), respectively. Relative recovery with 0.1, 1 and 10 mg/l (in blood) were 114.9%, 99.3% and 87.4% (n=6), respectively. The described method can be applied in forensic toxicology to determine strychnine in blood samples.
목차
등록된 정보가 없습니다.
참고문헌 (13)
참고문헌 신청
Moffat, A. C / 2011 / Clarke's Analysis of Drugs and Poisons in Pharmaceutical Body Fluids and Postmortem Material / Pharmaceutical Press
Baselt, R. C / 2011 / Disposition of Toxic Drugs and Chemicals in Man / Biomedical Publications, Chemical Toxicology Institute :1575
True, B. L / 2002 / Dreisbach's Handbook of Poisoning, Prevention, Diagnosis and Treatment / The Parthenon Publishing Group :513
Wang, Z / 2004 / Analysis of strychnine and brucine in postmortem specimens by RPHPLC: A case report of fatal intoxication / Journal of Analytical Toxicology 28:141
Nooy, M / 2009 / Strychnine, An Accidental Poisoning / Proceeding of Regional Australasian Toxicolgy Meeting :41
Baselt, R. C / 2011 / Disposition of Toxic Drugs and Chemicals in Man / Biomedical Publications, Chemical Toxicology Institute :1575
True, B. L / 2002 / Dreisbach's Handbook of Poisoning, Prevention, Diagnosis and Treatment / The Parthenon Publishing Group :513
Wang, Z / 2004 / Analysis of strychnine and brucine in postmortem specimens by RPHPLC: A case report of fatal intoxication / Journal of Analytical Toxicology 28:141
Nooy, M / 2009 / Strychnine, An Accidental Poisoning / Proceeding of Regional Australasian Toxicolgy Meeting :41
이 논문의 저자 정보
최근 본 자료
댓글(0)
0