지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Non-alcoholic fatty liver disease is known to be frequently associated with obesity and type 2 diabetes. We examined the effects of EtOH extracts from Triticum aestivum on lipid accumulation during the differentiation of 3T3-L1 preadipocytes to screening the candidate materials in preventing non-alcoholic fatty liver disease. The lipid level in adipocytes was determined by Oil Red O staining. The treatment of 50% ethanol, but not water and 100% ethanol extracts, from Triticum aestivum at concentration of 0.5 $mg/ml$ inhibited lipid accumulation in 3T3-L1 cells, revealing no cell toxicity. Thus, the fractions of $CH_2Cl_2$, EtOAc and BuOH were separated from 50% EtOH extract to characterize anti-adipogenic effect. The $CH_2Cl_2$ fraction at concentration of $50{\mu}g/ml$ effectively inhibited the lipid accumulation in the adipocytes compared to those of EtOAc and BuOH at concentration of $50{\mu}g/ml$. The intracellular triglyceride accumulation also was significantly reduced by treatment of $CH_2Cl_2$ fraction in concentration-dependent manner. Western blot analysis showed that the $CH_2Cl_2$ fraction attenuated the intracelluar level of fatty acid synthase(FAS) accompanied by attenuated expression of Peroxidase proliferator-activated receptor ${\gamma}$ ($PPAR{\gamma}$) adipogenic transcription factor. These results suggest that $CH_2Cl_2$ fraction from 50% EtOH extract of Triticum aestivum may has the potent anti-adipogenic effects by inhibiting the transactivation of $PPAR{\gamma}$.
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참고문헌 (22)
참고문헌 신청
Cortez-Pinto, H / 1999 / Nonalcoholic fatty liver: another feature of metabolic syndrome / Clin. Nutr 18:353
Levy, R. I / 1981 / Cholesterol, lipoproteins, apoproteins, and heart disease: present status and future prospects / Clin. Chem 27:653
Delgado, J. S / 2008 / Evolving trends in nonalcoholic fatty liver disease / Eur. J. Intern. Med 19:75
Frayn, K. N / 2003 / Integrative physiology of human adipose tissue / Int. J. Obes. Relat. Metab. Disord 27:875
Darlington, G. J / 1998 / The role of C/EBP genes in adipocyte differentiation / J. Biol. Chem 273:30057
Levy, R. I / 1981 / Cholesterol, lipoproteins, apoproteins, and heart disease: present status and future prospects / Clin. Chem 27:653
Delgado, J. S / 2008 / Evolving trends in nonalcoholic fatty liver disease / Eur. J. Intern. Med 19:75
Frayn, K. N / 2003 / Integrative physiology of human adipose tissue / Int. J. Obes. Relat. Metab. Disord 27:875
Darlington, G. J / 1998 / The role of C/EBP genes in adipocyte differentiation / J. Biol. Chem 273:30057
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