지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Simultaneous monitoring of catecholamines and serotonin with their appropriate extraction from the biological samples is required in order to understand thoroughly the regulation of the central and peripheral nervous system. In the present research the segmented gradient elution with the solid phase extraction using a C18 cartridge rather than the previous isocratic elution with alumina extraction is successfully employed to determine norepinephrine (NE), epinephrine (E), dopamine (DA), serotonin (5HT), 3,4-dihydroxyphenylacetic acid (DOPAC) and 5-hydroxyindoleacetic acid (5HIAA) simultaneously within 20 minutes using 3,4-dihydroxyhydrocinnamic aicd as the internal standard (IS). Linearities were obtained in the concentration range between $5{\times}10^{-6}M$ and $1{\times}10^{-4}M$ for all 7 compounds with detection limits of 0.6~1.9 ${\mu}M$. The present HPLC/ECD method yielded reasonable accuracy (relative error; -1.4~1.1%) and precision (relative standard deviation; 0.4~1.9%) for 9 measurements of the standard solution consisting of NE, E, DA, 5HT, DOPAC and 5HIAA compounds. Recoveries of catecholamines, serotonin and their metabolites from human serum were in the range of 57%~86%. While the concentrations of NE and 5HT in the serum of normal Sprague-Dawley rat were found as $1.4{\times}10^{-6}M$ and $2.6{\times}10^{-6}M$, respectively, the contents of NE and 5HT in the serum of the stressed rat were increased 5.6 times and 1.4 times more, respectively.
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참고문헌 (11)
참고문헌 신청
Kopin, I. J / 1985 / Catecholamine metabolism: basic aspects and clinical significance / Pharmacol. Rev 37:333
Thierry, A.-M / 1968 / Effects of stress on the metabolism of norepinephrine, dopamine, and serotonin in the central nervous system of the rat.I. modifications of norepinephrine turnover / J. Pharmacol. Exp. Ther 163:163
Raggi, M. A. / 1999 / Determination of catecholamines in human plasma by high-performance liquid chromatography with electrochemical detection / J. Chromatogr. B 730:201
Sastre, E / 2004 / Method for simultaneous measurement of norepinephrine, 3- methoxy-4-hydroxyphenylglycol and 3,4-dihydroxyphenylglycol by liquid chromatography with electrochemical detection: application in rat cerebral cortex and plasma after lithium chloride treatment / J. Chromatogr. B 801:205
Patel, B. A / 2005 / Simple and rapid determination of serotonin and catecholamines in biological tissue using high-performance liquid chromatography with electrochemical detection / J. Chromatogr 818:269
Thierry, A.-M / 1968 / Effects of stress on the metabolism of norepinephrine, dopamine, and serotonin in the central nervous system of the rat.I. modifications of norepinephrine turnover / J. Pharmacol. Exp. Ther 163:163
Raggi, M. A. / 1999 / Determination of catecholamines in human plasma by high-performance liquid chromatography with electrochemical detection / J. Chromatogr. B 730:201
Sastre, E / 2004 / Method for simultaneous measurement of norepinephrine, 3- methoxy-4-hydroxyphenylglycol and 3,4-dihydroxyphenylglycol by liquid chromatography with electrochemical detection: application in rat cerebral cortex and plasma after lithium chloride treatment / J. Chromatogr. B 801:205
Patel, B. A / 2005 / Simple and rapid determination of serotonin and catecholamines in biological tissue using high-performance liquid chromatography with electrochemical detection / J. Chromatogr 818:269
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