지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 제37권 제2호
- 발행연도
- 2014.1
- 수록면
- 239 - 244 (6page)
이용수
초록· 키워드
오류제보하기
This study was performed to investigate the effects of acteoside on various cellular functions such as, intracellular $Ca^{2+}$ mobilization, phospholipase C activity, and exocytosis induced by melittin. Melittin ($0.1-1{\mu}M$) dose-dependently increased intracellular $Ca^{2+}$ mobilization in the presence of extracellular $Ca^{2+}$, but was not affected by $1{\mu}M$ U73122, a specific PLC inhibitor. In the absence of extracellular $Ca^{2+}$, melittin ($1{\mu}M$) did not induce a change in intracellular $Ca^{2+}$ mobilization, which suggests that melittin-induced intracellular $Ca^{2+}$ mobilization may be dependent on the influx of extracellular $Ca^{2+}$ rather than on the release of intracellular $Ca^{2+}$ storage. Acteoside ($10{\mu}M$) significantly inhibited $1{\mu}M$ melittin-induced $Ca^{2+}$ mobilization by 33 %. In [$^3H$]inositol-labeled cells, $1{\mu}M$ melittin did not increase inositol phosphate formation, but more than $5{\mu}M$ melittin significantly increased inositol phosphate formation, which was significantly inhibited by acteoside. Melittin ($1{\mu}M$) significantly increased histamine release from RBL 2H3 cells in the presence or absence of extracellular$Ca^{2+}$. Acteoside significantly inhibited $1-{\mu}M$-melittin-induced histamine release by 74 % in the presence of extracellular $Ca^{2+}$ and by 71 % in the absence of extracellular $Ca^{2+}$. These data suggest that the inhibitory effect of acteoside on $1{\mu}M$-melittin-induced histamine release may be related to blockage of the calcium-independent pathway. Taken together, these data suggest that melittin has an influence on cellular functions such as intracellular $Ca^{2+}$ mobilization, the PLC pathway, and exocytosis via various independent signalling pathways in RBL-2H3 cells, and was significantly inhibited by acteoside.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청이 논문의 저자 정보
최근 본 자료
댓글(0)
0