지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Previously, we have reported that apigenin, a natural flavonoid found in a variety of vegetables and fruits, stimulated melanogenesis through the activation of $K^+-Cl^-$-cotransport (KCC) in B16 melanoma cells. In this study we investigated the possible involvement of reactive oxygen species (ROS) in the mechanism of apigenin-induced melanogenesis in B16 cells. Apigenin elevated intracellular ROS level in a dose-dependent manner. Treatment with various inhibitors of NADPH oxidase, diphenylene iodonium (DPI), apocynin (Apo) and neopterine (NP) significantly inhibited both the generation of ROS and melanogenesis induced by apigenin. In addition these inhibitors profoundly inhibited apigenin-induced $Cl^-$-dependent $K^+$ efflux, a hallmark of KCC activity. However, the apigenin-induced ROS generation was not significantly affected by treatment with a specific KCC inhibitor R-(+)-[(2-n-butyl-6,7-dichloro-2-cyclopentyl-2,3-dihydro-1-oxo-1H-inden-5-yl)oxy]acetic acid (DIOA). These results indicate that the ROS production may be a upstream regulator of the apigenin-induced KCC stimulation, and in turn, melanogenesis in the B16 cells. Taken together, these results suggest that the NADPH oxidase-mediated ROS production may play an important role in the apigenin-induced melanogenesis in B16 cells. These results further suggest that NADPH oxidase may be a good target for the management of hyperpigmentation disorders.
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참고문헌 (46)
참고문헌 신청
Seiberg, M / 2001 / Keratinocyte-melanocyte interaction during melanosome transfer / Pigment Cell Res 14:236
Coelho, S. G / 2009 / Short- and long-term effects of UV radiation on the pigmentation of human skin / J. Investig. Dermatol. Symp. Proc 14:32
Kondo, T / 2011 / Update on the regulation of mammalian melanocyte function and skin pigmentation / Expert Rev. Dermatol 6:97
Wang, H. M / 2011 / Identifying melanogenesis inhibitors from Cinnamomum subavenium with in vitro and in vivo screening systems by targeting the human tyrosinase / Exp. Dermatol 20:242
Peterson, J / 1988 / Flavonoids: dietary occurrence and biochemical activity / Nutr. Res 18:1995
Coelho, S. G / 2009 / Short- and long-term effects of UV radiation on the pigmentation of human skin / J. Investig. Dermatol. Symp. Proc 14:32
Kondo, T / 2011 / Update on the regulation of mammalian melanocyte function and skin pigmentation / Expert Rev. Dermatol 6:97
Wang, H. M / 2011 / Identifying melanogenesis inhibitors from Cinnamomum subavenium with in vitro and in vivo screening systems by targeting the human tyrosinase / Exp. Dermatol 20:242
Peterson, J / 1988 / Flavonoids: dietary occurrence and biochemical activity / Nutr. Res 18:1995
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