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논문 기본 정보

자료유형
학술저널
저자정보
조영근 (경성대학교 이과대학 생물학과 및 기초과학연구소)
저널정보
한국지하수토양환경학회 지하수토양환경 지하수토양환경 제17권 제1호
발행연도
2012.1
수록면
47 - 54 (8page)

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초록· 키워드

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When quicklime is added into soil for various purposes, abrupt changes in soil chemistry may affect essential ecological functions played by indigenous bacterial communities in soil. The magnitude of influence was estimated by observing changes in abundance and diversity of soil bacteria after quicklime treatment. When several soil samples were treated up to 20% (w/w) quicklime, plate count of viable cells ranged $10^2{\sim}10^3$ CFU $g^{-1}$, showing a reduction of more than $10^4$ times from viable counts of the untreated sample. Diversity of the bacterial isolates that survived after quicklime treatment was analyzed by conducting $GTG_5$ rep-PCR fingerprinting. There were only two types of fingerprints common to both 5% and 20% quicklime samples, implying that bacteria surviving at different strength of quicklime treatment differed depending on their tolerance to quicklime-treated condition. Isolates surviving the quicklime treatments were further characterized by Gram staining and endospore staining. All isolates were found to be Gram positive bacteria, and 85.4% of them displayed endospores state. In conclusion, most bacteria surviving quicklime treatment appear to be endospores. This finding suggests that most of ecological functions of bacteria in soil are lost with quicklime treatment.

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