지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 165 - 171 (7page)
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초록· 키워드
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Pseudomonas aeruginosa has been considered as a representative pathogenic bacteria in drinking water. In order to detect P. aeruginosa, aptamers were utilized in this study. In particular, fluorescein isothiocyannate (FITC) and quantum dot (QD) were used for aptamer labeling. FITC-labeled aptamers showed higher binding capacity with optimal incubation time of 30 min compared to QD-labeled aptamers. However, incubation speed did not have any effect on the binding capacity of FITC-labeled aptamers to bacteria. Aptamer-binding capacity was measured according to varying cell concentrations of 0, 10, 100, and 1000 cells/mL. As a result, the limit of detection, limit of quantification, and limit of linearity of P. aeruginosa were 5.07, 5.64, and 100 cells/mL, respectively. The low detection limit shows the fluorophore-labeled aptamer potential to detect P. aeruginosa labeling in the field.
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