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자료유형
학술저널
저자정보
Kim, Jin Young (Department of medicine, School of Medicine, Jeju National University) Kim, Yeon-Ki (GreenGene Biotech Inc., Myongji University) Kim, Young Mee (Department of medicine, School of Medicine, Jeju National University) Lee, Seogjae (Department of medicine, School of Medicine, Jeju National University) Park, Sanggyu (Division of Life & Environmental Science, Daegu University) Nahm, Baek Hie (GreenGene Biotech Inc., Myongji University) Lee, Dong-Sun (College of Applied Life Science [RISA], Jeju National University) Cho, Moonjae (Department of medicine, School of Medicine, Jeju National University)
저널정보
한국응용생명화학회 Applied Biological Chemistry Applied Biological Chemistry 제56권 제2호
발행연도
2013.1
수록면
157 - 163 (7page)

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Snail family proteins (Snail1 and Slug/Snail2) are transcription factors that regulate transcription of molecules during epithelial-mesenchymal transition (EMT). Snail1/2 is known to bind to the E-box motif (CANNTG). The proteasome activity is decreased in EMT (Kim et al., 2011), and several E-box motifs are found in the promoters of genes coding for proteasome subunits. We used a new protein-binding microarray to specify the Slug/Snail2 binding sequence. Among 563 9-mer clusters, the motif CACCTGC yielded the highest P-value in the Wilcoxon-Mann-Whitney test. Within this motif, the A and T were absolutely required, and CC was preferred, but could be replaced by GG with little effect. In hepatocytes overexpressing Slug/Snail2, the 20S proteasome expression and proteasome activity were decreased partly due to the down-regulation of proteasome subunit beta type 2 (PSMB2) and PSMB3 transcription.

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