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논문 기본 정보

자료유형
학술저널
저자정보
Kim, Byungsoo (Department of Data Science, Institute of Statistical Information, Inje University) Kim, Jiyeon (College of General Education, Inje University)
저널정보
한국응용생명화학회 Applied Biological Chemistry Applied Biological Chemistry 제56권 제3호
발행연도
2013.1
수록면
279 - 287 (9page)

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Optimization of culture conditions for the production of biosurfactant by Bacillus subtilis JK-1 was carried out using central composite rotatable design (CCRD) of response surface methodology (RSM). The variables selected for optimization of culture conditions were soluble starch, skim milk, $KNO_3$ concentrations, and temperature. Response surface analysis showed that the fitted quadratic model had adequately high $R^2$ value of 0.9882 and low significance probability of <0.0001, which indicated the statistical significance of the model. The optimum levels of each variable were determined to be as follows: 1.550% (w/v) soluble starch, 0.477% (w/v) skim milk, 0.096% (w/v) $KNO_3$ and $37.145^{\circ}C$. Through the statistical analysis, temperature and soluble starch were found to be the most significant factors, whereas skim milk and $KNO_3$ had less effect within the ranges investigated. Our results could be very helpful in the large-scale production of biosurfactant from B. subtilis JK-1.

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