지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 749 - 755 (7page)
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초록· 키워드
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Flavonoids are ubiquitous secondary metabolites in plants and possess a wide range of biological activities such as anti-oxidant, anti-microbial, and anti-cancer activities. As a food ingredient, flavonoids are also thought to have health-promoting and disease-preventing properties. Structural modifications of flavonoids can improve their biological activities. Methylation is a common flavonoid modification that is catalyzed by O-methyltransferases (OMTs). We isolated four putative OMTs from the miniature tomato cultivar Micro-Tom. One OMT (SlOMT3) was successfully expressed in E. coli and purified. Recombinant SlOMT3 was found to be a regiospecific flavonoid 3'/5'-O-methyltransferase. Biochemical parameters show that SlOMT3 has higher binding affinity and catalytic efficiency for quercetin and luteolin than for eriodictyol. This suggests that flavonols and flavones are preferable substrates for SlOMT3. Biotransformation is considered a promising method to modify flavonoid structures. Transgenic E. coli expressing SlOMT3 was used to modify flavonoid substrates and was found to efficiently convert quercetin, luteolin, and eriodictyol to isorhamnetin, chrysoeriol and homoeriodictyol, respectively.
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