지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 633 - 642 (10page)
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초록· 키워드
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Random amplification of polymorphic DNA (RAPD) and NMR techniques were used to differentiate and identify species of black raspberry (BR) of different geographic origins. BR leaf samples from five geographic origins were identified as Rubus japonicus and R. coreanus by RAPD. $^1H$-NMR analysis was also performed for BR fruit extracts from different geographic origins. Major compounds assigned in $^1H$-NMR spectra of BR fruits were amino acids, organic acids, sugars, phenolic acids, and purine derivatives. In addition, relative levels of total phenolic compounds, flavanols, flavonoids, and anthocyanins in the BR fruits were further analyzed. Hierarchical cluster analyses (HCA) based on the genetic (RAPD of leaf samples) and metabolic ($^1H$-NMR, total phenolic compounds, flavanols, flavonoids and anthocyanins of fruit samples) datasets were independently performed. The HCA dendrogram pattern derived from RAPD genetic fingerprinting of BR leaf samples matched that from the $^1H$-NMR data of BR fruit samples. This research demonstrates that metabolic profiling of BR fruit based on $^1H$-NMR is a promising method for differentiating BR fruits of various species and geographic origins.
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