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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 79 - 92 (14page)
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Objectives : The aim of this study was identification of the anti-oxidative and anti-inflammatory effects of Talmyung-san (TMS) in mouse macrophage, RAW264.7 cells. Methods : To identify the anti-oxidative effect of TMS, scavenging activities of DPPH radical, nitric oxide and peroxynitrite were measured in vitro. In RAW264.7 cells, DCFH-DA assay was conducted to examine the inhibitory effect of TMS on ROS production in response to lipopolysaccharide. And the productions of nitric oxide (NO), $PGE_2$ and pro-inflammatory cytokines were measured. The levels of COX-2, iNOS, nuclear NF-${\kappa}B$ p65 expression and phosphorylation of $I{\kappa}B-{\alpha}$ in cytosol were detected by western blotting analyses. Results : TMS couldn't scavenged DPPH radical, but nitric oxide and peroxynitrite were decreased. TMS decreased intracellular ROS, NO, and IL-$1{\beta}$ production effectively. However, TMS inhibited $PGE_2$ levels only in high concentration ($300{\mu}g/m{\ell}$) and TMS failed to suppress the production of IL-6 and TNF-${\alpha}$. Results from immunoblot analyses revealed that TMS decreased activation of COX-2, iNOS, phosphorylation of $I{\kappa}B-{\alpha}$ and nuclear translocation of p65. Conclusions : TMS has anti-RNS and anti-inflammatory effects via NF-${\kappa}B$ pathway and more intensive studies will be required to evaluate therapeutic potential of TMS.
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참고문헌 (39)
참고문헌 신청
Heo J. / 1999 / Treasured Mirror of Eastern Medicine / Bubin Publishers CO :954
Bag SH. / 2000 / Effects of Talmyungsan on the Cultured Rat Myocardiac Cell and Vascular Smooth Muscle Cell / J Korean Oriental Internal Medicine 21(1):46~54
Wu Qian. / 1991 / The Golden Mirror of Medicine / Daesung Publishers CO :339~341
Kim BY. / 2013 / Gene Expression Profile of the A549 Human Non-Small Cell Lung Carcinoma Cell Line following Treatment with the Seeds of Descurainia sophia, a Potential Anticancer Drug / Evid Based Complement Alternat Med 2013:584604
You Jin Lee / 2013 / Cytotoxic and anti-inflammatory constituents from the seeds of Descurainia sophia / Archives of Pharmacal Research 36(5):536~541
Bag SH. / 2000 / Effects of Talmyungsan on the Cultured Rat Myocardiac Cell and Vascular Smooth Muscle Cell / J Korean Oriental Internal Medicine 21(1):46~54
Wu Qian. / 1991 / The Golden Mirror of Medicine / Daesung Publishers CO :339~341
Kim BY. / 2013 / Gene Expression Profile of the A549 Human Non-Small Cell Lung Carcinoma Cell Line following Treatment with the Seeds of Descurainia sophia, a Potential Anticancer Drug / Evid Based Complement Alternat Med 2013:584604
You Jin Lee / 2013 / Cytotoxic and anti-inflammatory constituents from the seeds of Descurainia sophia / Archives of Pharmacal Research 36(5):536~541
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