지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 77 - 82 (6page)
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Objectives : The effects of ethanol extract of Plantago asiatica L. were investgated on adipocyte differentiation, lipopogenesis, lipolysis and apoptosis using differnentiated 3T3-L1 adipocytes. Methods : Plantago asiatica L. was extracted with ethanol (CCE). We carried on MTT assay for cell proliferation, Oil Red O staining for determination of cell differentiation and intracelluar adipogenesis. TUNEL staining assay for cell apoptosis, and Western blot analysis for measurement of pAMPK and pACC, $C/EBP{\alpha}$, $PPAR{\gamma}$ protein expressions were performed. Results : The addition of CCE up to 0.2 mg/ml into cell culture media showed no cytotoxicity. Treatment of 0.2 mg/ml CCE significantly inhibited differentiation in 3T3-L1 preadipocytes. Lipid accumulation of the CCE treated cells was decreased compared with that of control. Induction of cell apoptosis was increased in CCE treated cells compared with that of control. AMPK and ACC levels of the cells with 0.2 mg/ml CCE were led to phosphorylation and also expressions of $C/EBP{\alpha}$ and $PPAR{\gamma}$, as adipogenic transcription factors, were suppressed compared with those of control. Conclusions : Taken together, these results provide evidence that CCE has a regulatory role in lipid metabolism that is related to differentiation into adipocytes, adipogenesis and apoptosis.
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참고문헌 (32)
참고문헌 신청
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Sharrett AR / 2001 / Coronary heart disease prediction from lipoprotein cholesterol levels, triglycerides, lipoprotein(a), apolipoproteins A-I and B, and HDL density subfractions: the atherosclerosis risk in communities (ARIC) study / Circulation 104(10):1108~1113
Huh KB / 1990 / Symposium: recent progress in obesity research: pathogenesis of obesity / Kor J Soc Food Sci Nutr 23:333~336
Sjostrom L / 1994 / The sagirral diameter is a valid maker of the visceral adipose tissue volume / J Progress Obes 7:309~319
Sjostrom LV / 1992 / Morbidity of severely obese subject / Am J Clin Nutr 55:508~513
Sharrett AR / 2001 / Coronary heart disease prediction from lipoprotein cholesterol levels, triglycerides, lipoprotein(a), apolipoproteins A-I and B, and HDL density subfractions: the atherosclerosis risk in communities (ARIC) study / Circulation 104(10):1108~1113
Huh KB / 1990 / Symposium: recent progress in obesity research: pathogenesis of obesity / Kor J Soc Food Sci Nutr 23:333~336
Sjostrom L / 1994 / The sagirral diameter is a valid maker of the visceral adipose tissue volume / J Progress Obes 7:309~319
Sjostrom LV / 1992 / Morbidity of severely obese subject / Am J Clin Nutr 55:508~513
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