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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 21 - 26 (6page)
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Objectives : Taraxacum coreanum (TC) have been used as a traditional medicine to treat inflammatory diseases and anti-oxidant effect in Korea. However, the anti-inflammatory effect of TC water extract on lipopolysaccharide (LPS)-induced inflammation is not well-known. Therefore, this study was performed to identify the anti-inflammatory effect of TC on LPS induced inflammatory. Methods : RAW 264.7 cells were treated with 500 ng/mL of LPS. Water extracts of TC (0.1, 0.25, 0.5 mg/ml) was treated 1 h prior to LPS. Cell viability was measured by MTT assay. Levels of nitric oxide (NO) were measured with Griess reagent and pro-inflammatory cytokines were measured by enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (real-time PCR). We also examined molecular mechanisms such as mitogen-activated protein kinases (MAPKs) and nuclear factor-B ($NF-{\kappa}B$) activation by western blot. Results : Water Extract from TC itself did not have any cytotoxic effect in RAW 264.7 cells. TC treatment inhibited the production of NO production, and pro-inflamamtory cytokines such as interleukin (IL)-6 and $IL-1{\beta}$ on protein and mRNA levels. In addition, TC treatment inhibited the LPS-induced activation of MAPKs such as extracellular signal-regulated kinase1/2 (ERK1/2), p38 kinases (p38), c-Jun $NH_2$-terminal kinase (JNK) and $NF-{\kappa}B$. Conclusions : In summary, our result suggest that treatment of TC could reduce the LPS-induced inflammation. Thereby, TC could be used as a protective agent against inflammation. Also, this study could give a clinical basis that TC could be a drug or agent to prevent inflammation.
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참고문헌 (25)
참고문헌 신청
Behrens EM / 2008 / Macrophage activation syndrome in rheumatic disease: What is the role of the antigen presenting cell? / Autoimmun Rev 7(4):305~308
Lopez-Bojorquez LN / 2004 / Molecular mechanisms involved in the pathogenesis of septic shock / Arch Med Res 35(6):465~479
McDaniel ML / 1996 / Cytokines and nitric oxide in islet inflammation and diabetes / Proc Soc Exp Biol Med 211(1):24~32
김대희 / 2009 / LPS로 활성화된 대식세포에서 黃連解毒湯 물추출물의 염증매개물질 억제효과 / 대한본초학회지 24(4):39~47
Moncada S / 1991 / Endogenous nitric oxide：physiology, pathology and clinical relevance / Eur J Clin Ivest 21(4):361~374
Lopez-Bojorquez LN / 2004 / Molecular mechanisms involved in the pathogenesis of septic shock / Arch Med Res 35(6):465~479
McDaniel ML / 1996 / Cytokines and nitric oxide in islet inflammation and diabetes / Proc Soc Exp Biol Med 211(1):24~32
김대희 / 2009 / LPS로 활성화된 대식세포에서 黃連解毒湯 물추출물의 염증매개물질 억제효과 / 대한본초학회지 24(4):39~47
Moncada S / 1991 / Endogenous nitric oxide：physiology, pathology and clinical relevance / Eur J Clin Ivest 21(4):361~374
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