지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 67 - 73 (7page)
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This study was performed to assess the neuroprotective effects of methanolic extracts from sweet persimmon peel (PPE) against glutamate-induced cytotoxicity in hybridoma N18-RE-105 cells. The neuroprotective effects of PPE in N18-RE-105 cells were measured using the MTT reduction assay, LDH release assay, and phase-contrast microscopy. The results of the MTT reduction assay showed that treating cells with 500 ${\mu}g/ml$ PPE resulted in cell viability of 66.9%. Additionally, the morphological changes and the results of the LDH release assay showed that glutamate-induced damage to nerve cells was strongly inhibited by PPE. GSH content of N18-RE-105 cells was 3.5 ${\mu}M$ compared to that of the control, whereas pretreatment with 500 ${\mu}g/ml$ PPE increased GSH content by 4.7 ${\mu}M$. PPE was fractionated with hexane, and that layer had the highest neuroprotective effects in glutamate-stressed N18-RE-105 cells. In conclusion, our data showed that glutamate potentiated the effects of N18-RE-105 cell death by a mechanism involving oxidative stress. Therefore, PPE may be a potential candidate for prevention and therapy of neurodegenerative diseases.
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참고문헌 (32)
참고문헌 신청
Ames BN / 1981 / Uric acid provides an antioxidant defense in humans against oxidant- and radical-caused aging and cancer: A hypothesis / Proc Natl Acad Sci USA 78(11):6858~6862
Ansari MA / 2009 / Protective effect of quercetin in primary neurons against Abeta 1-42 relevance to Alzheimer's disease / J Nutr Biochem 20(4):269~275
Bhandari U / 2008 / Protective effect of aqueous extract of Embelia ribes Burm fruits in middle cerebral artery occlusion induced focal cerebral ischemia in rats / Indian J Pharmacol 40(5):215~220
Celik A / 2008 / Persimmon cv Hachiya Diospyros kaki Thunb fruit some physical chemical and nutritional properties / Int J Food Sci Nutr 59(7):599~606
Cheng HY / 2008 / Schizandrin protects primary cultures of rat cortical cells from glutamate induced excitotoxicity / J Pharmacol Sci 107(1):21~31
Ansari MA / 2009 / Protective effect of quercetin in primary neurons against Abeta 1-42 relevance to Alzheimer's disease / J Nutr Biochem 20(4):269~275
Bhandari U / 2008 / Protective effect of aqueous extract of Embelia ribes Burm fruits in middle cerebral artery occlusion induced focal cerebral ischemia in rats / Indian J Pharmacol 40(5):215~220
Celik A / 2008 / Persimmon cv Hachiya Diospyros kaki Thunb fruit some physical chemical and nutritional properties / Int J Food Sci Nutr 59(7):599~606
Cheng HY / 2008 / Schizandrin protects primary cultures of rat cortical cells from glutamate induced excitotoxicity / J Pharmacol Sci 107(1):21~31
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