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논문 기본 정보

자료유형
학술저널
저자정보
Hyerim Han (Horticultural Environment Division, National Horticultural Research Institute, RDA) Cho, Myoung-Rae (Horticultural Environment Division, National Horticultural Research Institute, RD) Jeon, Heung-Yong (Horticultural Environment Division, National Horticultural Research Institute, RD) Lim, Chun-Keun (Division of Biological Environment, College of Agriculture and Life Sciences, Kangwon National Universit) Jang, Han-Ik (Horticultural Environment Division, National Horticultural Research Institute, RDA)
저널정보
한국응용곤충학회 Journal of Asia-Pacific Entomology Journal of asia-pacific entomology 제7권 제2호
발행연도
2004.1
수록면
171 - 175 (5page)

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초록· 키워드

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Partial mitochondrial DNA from single female or second stage juvenile (J2) of root-knot nematodes was amplified by PCR (polymerase chain reaction), and the further analysis by RFLP (restriction fragment length polymorphism) provided discriminatory profiles useful for three major Meloidogyne species, M arenaria, M incognita, and M hapla, in Korea. The nematode DNA was extracted individually in nuclease-free water. The sizes of PCR product (1.7 kb and 500 bp) and restriction patterns obtained from single female nematodes were consistent with the results from single J2 within the same species. M. hapla was easily differentiated from the two other root-knot nematode species by the size of the PCR products. A fragment of 500 bp was generated from M. hapla, while M. arenaria, and M. incognita produced an 1.7 kb fragment in PCR amplification. M arenaria could be distinguished from M. incognita by analysis of restriction enzyme digestion with HinfI or Alu I. Hinf I had no digestion site in mitochondrial DNA of M. arenaria; however, it generated 1.3 kb and 400 bp fragments in M. incognita. Alu I digestion resulted in 1 kb, 460 bp, and 250 bp fragments in M. arenaria, but showed different digestion patterns in M. incognita by generating 800 bp, 460 bp, 250 bp, and 150 bp fragments.

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