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논문 기본 정보

자료유형
학술저널
저자정보
Goo, Yang-Mo (Department of Pharmacy, Seoul National University) Lim, Hyon-Joo (Department of Pharmacy, Seoul National University) Lim, Seok-Ran (Department of Pharmacy, Seoul National University) Kim, Kong-Hwan (Department of Biotechnology, Ajou University) Lim, Bun-Sam (ResearchandDevelopment Center) Lee, Sae-Bae (ResearchandDevelopment Center)
저널정보
대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 제12권 제4호
발행연도
1989.1
수록면
249 - 253 (5page)

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High producers or blocked mutants of aminoglycoside antibiotic-producing Streptomyces spp. were selected by application of an agar plug method and by culturing individual colonies in broth. The productivities of aminoglycoside antibiotic producing organisms were increased by selection of a high producer from colonies obtained by spreading spores of wild strain, or survived from treatment of a mutagen or from the colonies regenerated from protoplast-formation and cell-wall regenerations. Some mutagen treated colonies lost the ability to produce antibiotics (5-8%). Some A-factor negative and deostreptamine or streptidine negative mutants were obtained by N-methyl-N'-nitro-N-nitrosomethylguanidine (MNNG) treatment. Many of the survivors from the MNNG treatment lost the ability to produce antibiotics. Major colonies produced less amount of antibiotics ; only few survived colonies produced more antibiotics than the parent. Resistance of Streptomyces spp. against the antibiotics produced by itself was also markedly affected by mutagen treatment.

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