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논문 기본 정보

자료유형
학술저널
저자정보
Jeon, Young-Jae (Department of Microbiology and Institute of Basic Sciences, Dankook University) Kwon, Hyuk-Woo (Department of Microbiology and Institute of Basic Sciences, Dankook University) Nam, Ji-Sun (Department of Microbiology and Institute of Basic Sciences, Dankook University) Kim, Seong-Hwan (Department of Microbiology and Institute of Basic Sciences, Dankook University)
저널정보
한국균학회 Mycobiology Mycobiology 제34권 제3호
발행연도
2006.1
수록면
154 - 157 (4page)

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초록· 키워드

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A fungal isolate collected from infected paprika (Capsicum annuum var. grossum) was characterized as Sclerotinia sclerotiorum based on its ability of sclerotium formation, physiological and molecular properties. When the isolate was grown on potato dextrose agar, oatmeal agar, and malt extract agar, it grew most well on PDA. Optimal temperature and pH for its growth were $25^{\circ}C$ and pH 7, respectively. The fungal isolate produced sclerotia on PDA within 10 days, and the color and shape of the sclerotia were similar to those of S. sclerotiorum. The ITS rDNA regions including ITS1 and ITS2 and 5.8S sequences were amplified using ITS1F and ITS4 primers from the genomic DNAs of the paprika isolate and other known pathogenic S. sclerotiorum isolated from different crops in Korea, and their nucleotide sequences were determined. Sequence comparison analysis showed the ITS rDNA of the paprika isolate shares 100% sequence identity with those of S. sclerotiorum isolated from red pepper, lettuce and a S. sclerotiorum isolate registered in GenBank DNA database. Neighbor joining analysis based on the ITS rDNA sequence revealed the paprika isolate has very close phylogenetic relationships with known Sclerotinia sclerotiorum isolates. This is the first report that S. sclerotiorum has been found associated with paprika rot in paprika growing countries.

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