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자료유형
학술저널
저자정보
P, Vijayan (Department of Pharmaceutical Biotechnology, J.S.S. College of Pharmacy) HC, Prashanth (Department of Pharmaceutical Biotechnology, J.S.S. College of Pharmacy) Vijayaraj, Preethi (Department of Pharmaceutical Biotechnology, J.S.S. College of Pharmacy) H, Raghu Chandrashekhar (Department of Pharmaceutical Biotechnology, J.S.S. College of Pharmacy) Godavarthi, Ashok (Department of Pharmaceutical Biotechnology, J.S.S. College of Pharmacy) SA, Dhanaraj (Department of Pharmaceutical Biotechnology, J.S.S. College of Pharmacy)
저널정보
경희한의학연구센터 Oriental pharmacy and experimental medicine Oriental pharmacy and experimental medicine 제6권 제1호
발행연도
2006.1
수록면
45 - 52 (8page)

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The methanol extracts of the roots, root bark and stem of Berberis tinctoria, were investigated for their hepatoprotective activity against carbon tetrachloride $(CCl_4)$ induced toxicity in freshly isolated rat hepatocytes, HEp-G2 cells and animal models. The methanol extracts were able to significantly normalise the levels of aspartate amino transferase, alanine aminotransferase, alkaline phosphatase, triglycerides, total proteins, albumin, total bilirubin and direct bilirubin, which were altered due to $CCl_4$ intoxication in freshly isolated rat hepatocytes and also in animal models. The anti-hepatotoxic effect of the methanol extracts in vitro were observed at $600\;-\;1,000\;{\mu}g/ml$ concentrations. A dose dependent increase in the percentage viability was observed when $CCl_4$ exposed HEp-G2 cells were treated with different concentrations of the methanol extracts. The highest percentage viability of HEp-G2 was observed at a concentration of $1,000\;{\mu}g/ml$. The results from the present investigations also indicate good correlation between the in vivo and in vitro studies.

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