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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Cymbopogon nardus L. could be propagated via tissue culture using axillary buds as explants. The aseptic bud explants obtained using double sterilization methods produced stunted abnormal multiple shoots when they were cultured on Murashige and Skoog medium (MS) supplemented with 1.0mg $L^{-1}$ or 2.0mg $L^{-1}$ benzyladenine (BA). Stunted shoots that cultured on MS + 1.0 mg $L^{-1}$ BA+1.0 mg $L^{-1}$ $N^6-isopentenyl-adenine$ (2iP) could induce elongation of shoots from about $60\%$ of the stunted shoots. Normal multiple shoots could be induced at the highest (19.7 shoots per bud) from the bud explahts within six weeks when cultured on proliferation medium consisted of MS supplemented with 0.3 mg $L^{-1}$ BA and 0.1 mg $L^{-1}$ indole-3-butyric acid (IBA). The separated individual shoot produced roots when transferred to basic MS solid medium. The essential oils that were contained in the mature plants namely citronellal, geraniol and citronellol were also found in the in vitro C. nardus plant-lets. Citronellal was the main essential oil component in the matured plants while geraniol was the main component in the in vitro plantlets.
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