지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Small heat-shock proteins (sHsps) are ubiquitous stress proteins with molecular chaperone activity. They share characteristic homology with the $\alpha-crystallin$ protein of the mammalian eye lens as well as being ATP. independent in their chaperone activity. We isolated a clone for a cytosolic class I sHsp, NtHSPI7.6, from Nicotiana tabacum, and analyzed its functional mode for such activity. Following its transformation into Escherichia coli and its over-expression, NtHSP17.6 was purified and examined in vitro. This purified NtHSP17.6 exhibited typical chaperone activity in a lightscattering test It was enable to protect a model substrate, firefly luciferase, from heat-induced aggregation. Nondenaturing PAGE showed that NtHSP17.6 formed a dodecamer in its native conformation, and was bound to its substrate under heat stress. A labeling test with bis-ANS indicated that this binding might be linked to newly exposed hydrophobic sites of the NtHSP17.6 complexes during heat shock. Based on these data, we suggest that NtHSP17.6 is a molecular chaperone that functions as a dodecamer in a heat-induced manner.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Molecular Cloning and Expression Pattern Analyses of Heat Shock Protein 70 Genes from Nicotiana tabacum
식물학회지
2004 .01
-
Molecular Chaperone Activity of a Tobacco Small Heat Shock Protein, NtHSP18.3.
한국동물학회 학술대회
2003 .01
-
Overexpression of NtHSP70-1 Protects Chlorophyll from High Temperature in Plants
생명과학회지
2008 .03
-
Analysis of Thermoprotective Activity Induced by Heat Shock Protein 70 in Plants Using Two-Dimensional Difference Gel Electrophoresis
생명과학회지
2008 .05
-
Genes and Expression Pattern of Tobacco Mitochondrial Small Heat Shock Protein under High-Temperature Stress
식물학회지
2003 .01
-
Screening Molecular Chaperones Similar to Small Heat Shock Proteins in Schizosaccharomyces pombe
Mycobiology
2015 .01
-
Inhibition of Citrate Synthase Thermal Aggregation In Vitro by Recombinant Small Heat Shock Proteins
Journal of Microbiology and Biotechnology
2009 .01
-
The HSP90-SGT1-RAR1 Molecular Chaperone Complex: a Core Modulator in Plant Immunity
식물학회지
2008 .01
-
세포내인자로서의 정교한 기능을 하는 molecular chaperone ( Molecular chaperone as a sophisticated intracellular membership )
생명과학회지
1998 .04
-
Organization and Expression Pattern of Class I Low Molecular Weight Heat-Shock Proteins in Tobacco
한국식물학회 학술발표대회
2000 .01
-
Substrate-binding Mechanism of Yeast Molecular Chaperone Hsp40
한국미생물학회 학술대회논문집
2005 .05
-
Age-related decline in expression of calnexin
Experimental and Molecular Medicine
2004 .01
-
Rescue of Oxidative Stress by Molecular Chaperones in Yeast
한국미생물학회 학술대회논문집
2001 .10
-
Effects of a mild heat treatment on mouse testicular gene expression and sperm quality
Animal cells and systems
2010 .01
-
Increased Heat Resistance of Geobacillus stearothermophilus Spores Heat-Shocked During Sporulation
Journal of Microbiology and Biotechnology
2006 .01
-
Expression of Heat Shock Protein 70 in Human Endothelial Cells with Hemodynamic Shear Stress.
한국동물학회 학술대회
1996 .01
-
Involvement of Putative Heat Shock Element in Transcriptional Regulation of $p21^{WAF1/ClP1/SDl1}$ by Heat Shock
Korean journal of biological sciences
2000 .01
-
Effects of Chaperones on mRNA Stability and Gene Expression in Escherichia coli
Journal of Microbiology and Biotechnology
2008 .01
-
소포체 molecular chaperone ERp29의 기능연구
한국생명과학회 심포지움
2003 .05
-
Bioinformatics Analysis of Hsp20 Sequences in Proteobacteria
Genomics & informatics
2009 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0