The Isolation and Antioxidative Effects of Vitexin from Acer palmatum

Kim Jin Hwa; Lee Bum Chun; Kim Jin Hui; Sim Gwan Sub; Lee Dong Hwan; Lee Kyung Eun; Yun Yeo Pyo; Pyo Hyeong Bae

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자료유형: 학술저널

저자정보: Kim Jin Hwa (R&D Center, Hanbul Cosmetics Co. Ltd.) Lee Bum Chun (R&D Center, Hanbul Cosmetics Co. Ltd.) Kim Jin Hui (R&D Center, Hanbul Cosmetics Co. Ltd.) Sim Gwan Sub (R&D Center, Hanbul Cosmetics Co. Ltd.) Lee Dong Hwan (R&D Center, Hanbul Cosmetics Co. Ltd.) Lee Kyung Eun (R&D Center, Hanbul Cosmetics Co. Ltd.) Yun Yeo Pyo (College of Pharmacy, Chungbuk National University) Pyo Hyeong Bae (R&D Center, Hanbul Cosmetics Co. Ltd. College of Pharmacy, Chungbuk National University)

저널정보: 대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 제28권 제2호

발행연도: 2005.1

수록면: 195 - 202 (8page)

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Free radicals and reactive oxygen species (ROS) caused by UV exposure or other environmental factors are critical players in cellular damage and aging. In order to develop a new antiphotoaging agent, this work focused on the antioxidant effects of the extract of tinged autumnal leaves of Acer palmatum. One compound was isolated from an ethyl acetate soluble fraction of the A. palmatum extract using silica gel column chromatography. The chemical structure was identified as apigenin-8-C-beta-D-glucopyranoside, more commonly known as vitexin, by spectral analysis including LC-MS, FT-IR, UV, $^{1}H-$, and $^{13}C-NMR$. The biological activities of vitexin were investigated for the potential application of its anti-aging effects in the cosmetic field. Vitexin inhibited superoxide radicals by about $70\%$ at a concentration of $100\;{\mu}g/mL$ and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals by about $60\%$ at a concentration of $100\;{\mu}g/mL$. Intracellular ROS scavenging activity was indicated by increases in dichlorofluorescein (DCF) fluorescence upon exposure to UVB $20\;mJ/cm^2$ in cultured human dermal fibroblasts (HDFs) after the treatment of vitexin. The results show that oxidation of 5-(6-)chloromethyl-2',7'-dichlo-rodihydrofluorescein diacetate ($CM-H_{2}DCFDA$) is inhibited by vitexin effectively and that vitexin has a potent free radical scavenging activity in UVB-irradiated HDFs. In ROS imaging using a confocal microscope we visualized DCF fluorescence in HDFs directly. In conclusion, our findings suggest that vitexin can be effectively used for the prevention of UV-induced adverse skin reactions such as free radical production and skin cell damage.

#Acer palmatum #Vitexin #Antioxidant #Free radical #Photoprotection

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