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자료유형
학술저널
저자정보
Iseki, Mineo (National Institute for Basic Biology,Bio-oriented Technology Research Advancement Institution,Center for Gene Research, Nagoya University) Matsunaga, Shigeru (Institute of Biological Sciences, University of Tsukuba) Murakami, Akio (Kobe University Research Center for Inland Seas) Ohno, Kaoru (National Institute for Basic Biology) Shiga, Kiyoshi (Department of Physiology, Kumamoto University School of Medicine) Yoshida, Kazuichi (National Institute for Basic Biology) Sugai, Michizo (Department of Biology, Faculty of Science, Toyama University) Takahashi, Tetsuo (School of Pharmaceutical Sciences, Toho University) Hori, Terumitsu (Institute of Biological Sciences, University of Tsukuba) Watanabe, Masakatsu (National Institute for Basic Biology, Dept. of Photoscience, School of Advanced Sciences, Graduate University)
저널정보
한국광과학회 Journal of photoscience : an international journal officail organ of the korean society of photoscience Journal of photoscience : an international journal officail organ of the korean society of photoscience 제9권 제2호
발행연도
2002.1
수록면
98 - 101 (4page)

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Euglena gracilis abruptly changes its swimming direction after a sudden increase or decrease in incident light intensity, that is, step-up or step-down photophobic responses, resulting in photoavoidance or photoaccumulation, respectively. To identify the photoreceptor molecules for these UV-A/blue-light type photobehaviors, we purified a flavoprotein from isolated putative photosencory organelles (PFBs) of Euglena. The purified flavoprotein, which noncovalently bound flavin adenine dinucleotide (FAD), seemed to be a heterotetramer of alpha- and beta-subunits. Predicted amino acid sequences of each of the subunits were similar to each other and contained two FAD-binding domains each followed by an adenylyl cyclase catalytic domain. The purified flavoprotein actually showed adenylyl cyclase activity, being drastically elevated by blue-light irradiation. Suppression of gene expression of the flavoprotein (Photoactivated Adenylyl Cyclase, PAC) by RNA interference (RNAi) caused loss of the step-up photophobic response, demonstrating that PAC actually mediates photoavoidance of Euglena.

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