지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2001.1
- 수록면
- 153 - 156 (4page)
이용수
초록· 키워드
오류제보하기
A key aspect of genomic research in the “post-genome era”is to associate sequence variations with heritable phenotypes. The most common variations in the human genome are single nucleotide polymorphisms (SNPs) that occur approximately once in every 500 to 1,000 bases. Although analyzing the phenotypic outcome of these SNPs is crucial to facilitate large-scale association studies of genetic diseases, detection of SNPs from an extended number of human DNA samples is often difficult, labor-intensive and time-consuming. Recent development in SNP detection methods using DNA microarrays and mass spectrophotometry has allowed automated high throughput analyses, but such equipments are not accessible to many scientists. In this study, we demonstrate that a simple PCR-based method using primers with a mismatched base at the 3'-end provides a fast and easy tool to identify known SNPs from human genomic DNA in a regular molecular biology laboratory. Results from this PCR amplification of specific alleles (PASA) analysis efficiently and accurately typed the Q576R polymorphism of human IL4 receptor from the genomic DNAs of 29 Koreans, including 9 samples whose genotype could not be discerned by the conventiona1 PCR-SSCP (single strand conformation polymorphism) method. Given the increasing attention to disease-associated polymorphisms in genomic research, this alternative technique will be very useful to identify SNPs in large-scale population studies.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Characterization of Single Nucleotide Polymorphisms in 55 Disease-Associated Genes in a Korean Population
Genomics & informatics
2007 .01
-
Investigation of Single Nucleotide Polymorphisms in Porcine Candidate Genes for Economic Traits in the Commercial Pig Breed
생명과학회지
2008 .06
-
Evaluation of Methods to Analyze SNP-based Association Studies in a DNA-Pooling Experiment with Preferential Amplification
한국생물정보시스템생물학회 학술대회
2005 .01
-
Gemoetrical verification of protein structure for single nucleotide polymorphism (SNP)
한국생물정보시스템생물학회 학술대회
2005 .01
-
Single Nucleotide Polymorphisms Associated with Asthma
한국유전체학회 Conference
2003 .01
-
Investigation of Single Nucleotide Polymorphisms in Porcine Candidate Gene for Growth and Meat Quality Traits in the Berkshire Breed
생명과학회지
2007 .12
-
Extension of a 5'- or 3'-end Genomic DNA Sequence by a Single PCR Amplification
조선자연과학논문집
2008 .01
-
Single nucleotide polymorphisms and variable number of tandem repeat polymorphism of CYP2E1 in Koreans
한국동물학회 학술대회
2001 .01
-
Single Nucleotide Polymorphisms associated with Asthma Phenotypes
한국유전체학회 Conference
2002 .01
-
Single Nucleotide Polymorph isms of a 16 kb Region on Human Chromosome 11 p15.5 that Includes the H19 Gene
Genomics & informatics
2005 .01
-
A Statistical Analysis of SNPs, In-Dels, and Their Flanking Sequences in Human Genomic Regions
Genomics & informatics
2007 .01
-
Characterization of Single Nucleotide Polymorphisms in Candidate Gene from Swine
한국생명과학회 심포지움
2008 .10
-
Allelic Frequencies of 20 Visible Phenotype Variants in the Korean Population
Genomics & informatics
2013 .01
-
Single Nucleotide Polymorphisms of the Matrix Metalloproteinase 1, 3, 7, 9 and 12 in Koreans
한국동물학회 학술대회
2003 .01
-
Acute exposure to silica nanoparticles aggravate airway inflammation: different effects according to surface characteristics
Experimental and Molecular Medicine
2015 .01
-
단감 품종 판별을 위한 single nucleotide polymorphism 마커 적용 검정
생명과학회지
2020 .07
-
-34 C>T single nucleotide polymorphism and variable number of tandem repeat polymorphism of Interleukine 4 in Koreans
한국동물학회 학술대회
2001 .01
-
Single Nucleotide Polymorphisms in the Non-coding region of CYP2E1 among Koreans
한국동물학회 학술대회
2000 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0