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논문 기본 정보

자료유형
학술저널
저자정보
Kim, Gi-Young (Department of Microbiology, College of Natural Sciences, Pusan National University) Lee, Goang-Jae (Research Center, Hansung Enterprise CO. LTD.) Ha, Myung-Gyu (Korea Basic Science Institute) Lee, Tae-Ho (Department of Microbiology, College of Natural Sciences, Pusan National University) Lee, Jae-Dong (Department of Microbiology, College of Natural Sciences, Pusan National University)
저널정보
한국균학회 Mycobiology Mycobiology 제28권 제1호
발행연도
2000.1
수록면
11 - 16 (6page)

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초록· 키워드

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The nucleotide sequences of the internal transcribed spacer (ITS) regions of the ribosomal DNA including the 5.8S ribosomal RNA gene (rDNA) have been determined for 11 species in order to analyze their intrageneric relationships. The total length of these sequences ranged from 530 nucleotides for Trichoderma reesei KCTC 1286 to 553 nucleotide for Trichoderma koningii IAM 12534. Generally speaking, the length of ITS1 region was about 30 nucleotides longer than that of the ITS2 region. Also, the sequences of 5.8S rDNA were more conserved in length and variation than those of ITS regions. Although the variable ITS sequences were often ambiguously aligned, the conserved sites were also found. Thus, a neighbor-joining tree was constructed using the full sequence data of the ITS regions and the 5.8S rDNA. The Trichoderma genus used to be grouped on the basis of the morphological features and especially the shape of phialides needs to be reexamined. The phylogenetic tree displayed the presence of monophylogeny in the species of Trichoderma. Therefore, it was difficult to distinguish the intrageneric relationships in the Trichoderma genus.

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