지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
For establishing a transformation system of rice (Oryza sativa), after three days of culture embryogenic suspension-cultured cell clusters were enzymatically macerated for 2 hours in electroporation buffer containing 2% cellulase and filtered through 550, 400, 250 and 100 \ulcorner stainless mesh. Filtered embryogenic microcolonies of 100-250 \ulcorner with pBI121 were electroporated at 400 V/cm for 1.2 ms. Four weeks after the electroporation, stable transformed calli were obtained at a frequency of 72% on the selection medium containing 100 mg/L kanamycin. GUS gene in the genomic DNA among 20 out of 22 putative transformed calli lines were detected by PCR analysis. The expression of GUS gene into the kanamycin-resistance calli was confirmed by spectrophotometric assay and histochemical assay of GUS activity. In ahistochemical study of the transgenic rice regenerants, it was shown that the GUS activity directed by the CaMV 35S promoter was localized mainly in leaf vein and root apex.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Development of a Screening System for Plant Defense-Inducing Agent using Transgenic Tobacco Plant with PR-la Promoter and GUS Gene
The Plant Pathology Journal
2005 .09
-
벼 원형질체의 분리, 배양 및 Electroporation에 관한 연구
식물학회지
1991 .01
-
Introduction and Expression of Foreign Genes in Rice Cells by Particle Bombardment
식물학회지
1994 .01
-
Expression Patterns of CaMV 35S Promoter-GUS in Transgenic Poatoes and Their Clonal Progenies
식물학회지
1994 .01
-
벼 진탕 배 배양세포로부터 원형질체 분리 및 배양
식물학회지
1988 .01
-
벼 종자 유래 배에서 외래유전자의 도입과 발현
식물학회지
1994 .01
-
Protocorm-like body를 이용한 호접란 형질전환 연구
생명과학회지
2009 .03
-
Optimal salt concentration of vehicle for plasmid DNA enhances gene transfer mediated by electroporation
Experimental and Molecular Medicine
2002 .01
-
Tissue Specific Expression of Wound-Inducible RCaM-2 Promoter in Transgenic Tobacco Plants
생명과학회지
2005 .04
-
Transient Gus Expression in Callus and OsMADS1 Gene Transformation Using Organogenesis from Explants of Seeds in Hot Pepper (Capsicum annuum L.)
한국동물학회 학술대회
1999 .01
-
Optimal Condition for Efficient DNA Transfer in Filamentous Cyanobacteria by Electroporation
The Journal of Microbiology
1997 .09
-
Electroporation에 의한 Escherichia coli-Lactobacillus casei 셔틀 벡터의 형질전환
미생물학회지
2000 .06
-
The increased GUS gene inactivation over generation in Arabidopsis transgenic lines
생명과학회지
2002 .02
-
Cloning and Expression of β-Glucuronidase from Lactobacillus brevis in E. coli and Application in Bioconversion of Baicalin and Wogonoside
Journal of Microbiology and Biotechnology
2009 .01
-
Thidiazuron Required for Efficient Somatic Embryogenesis from Suspension-Cultured Cells of Pimpinella brachycarpa
식물학회지
2001 .01
-
Efficient Transformation of Korean Rice Cultivars (Oryza sativa L.) Mediated by Agrobacterium tumefaciens
식물학회지
1998 .01
-
Improved in vitro Regeneration of Potato (Solanum tuberosum cv. Superior) Transformed by Agrobacterium Expressing $\beta-Glucuronidase$
식물학회지
1996 .01
-
Plant Regeneration from Shoot Tip-Derived Embryogenic Callus of Dianthus superbus
식물학회지
1994 .01
-
Potential Biochemical Markers for Somatic Embryos of Eurycoma Iongifolia Jack
식물학회지
2006 .01
-
The First Intron of Petunia Actin-Depolymerizing Factor Gene Enhances GUS Expression in Transgenic Arabidopsis
한국동물학회 학술대회
2001 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0