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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 1999.1
- 수록면
- 173 - 180 (8page)
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초록· 키워드
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A blue biliprotein, insecticyanin (INS), has been purified from the last instar larval hemolymph of Agrius convolvuli by ultracentrifugation, Sephadex G-100 gel permeation chromatography, and preparative electrophoresis. The molecular mass of INS was estimated to be 26 kDa and the N-terminal sequence of INS revealed high similarity to that of Manduca sexta. Results of Western blotting and autoradiography indicated that INS is synthesized by the epidermis and released into the hemolymph. In contrast to the INS reported in other insects, Agrius convolvuli INS contained a small amount of lipid, predominately consisting of triacylglycerol. Subcellular localization of INS was determined using protein-A gold particles linked to secondary antibodies (anti-rabbit Ig). INS was heavily accumulated in the cytoplasmic inclusion body (CIB). CIBs showed a variety of shapes from rod to globule and generally surrounded the nucleus. They were mostly located near the basement membrane and especially abundant in the intersegmental membrane.
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Purification and Characterization of Insecticyanin from the Hemolymph of the Agrius convolvuli
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Neuronal Cell Death of Sweet Potato Hornworm, Agrius convolvuli during Development
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Cloning and Prokaryotic Expression of C-type Lysozyme Gene from Agrius convolvuli
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Purification and Characterization of Ferritin from Sweet Potato Hornworm, Agrius convolvuli (Lepidoptera)
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Comparative Study on Characteristics of Lysozymes from the Hemolymph of Three Lepidopteran Larvae, Galleria mellonella, Bombyx mori, Agrius convolvuli
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Localization and Process Pathway of Allatostatin in the Central Nervous System of Lepidoteran Moth, Agrius Convolvuli
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Localization of Allatostatin - Producing Cells in Larval Gut of the Potato Worm Agrius convolvuli
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Purification and Properties of ApoLp-III from the Larval Hemolymph of Agrius convolvuli
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Purification and Activation of Prophenoloxidase from the Larval Hemolymph of Agrius convolvuli
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