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논문 기본 정보

자료유형
학술저널
저자정보
Chin Chur (Department of Biochemistry and Molecular Biology, College of Medicine, Pusan National University) Chung Byung-Seon (Department of Biochemistry and Molecular Biology, College of Medicine, Pusan National University)
저널정보
한국유전체학회 Genomics & informatics Genomics & informatics 제4권 제1호
발행연도
2006.1
수록면
11 - 15 (5page)

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초록· 키워드

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The aim of this study is to identify hRad21-binding sites in human chromosome, the core component of cohesin complex that held sister chromatids together. After chromatin immunoprecipitation with an hRad21 antibody, it was cloned the recovered DNA and sequenced 30 independent clones. Among them, 20 clones (67%) contained repetitive elements including short interspersed transposable elements (SINE or Alu elements), long terminal repeat (LTR) and long interspersed transposable elements (LINE), fourteen of these twenty (70%) repeats clones had Alu elements, which could be categorized as the old and the young Alu Subfamily, eleven of the fourteen (73%) Alu elements belonged to the old Alu Subfamily, and only three Alu elements were categorized as young Alu subfamily. There is no CpG island within these selected clones. Association of hRad21 with Alu was confirmed by chromatin immunoprecipitation-PCR using conserved Alu primers. The primers were designed in the flanking region of Alu, and the specific Alu element was shown in the selected clone. From these experiments, it was demonstrated that hRad21 could bind to SINE, LTRs, and LINE as well as Alu.

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