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자료유형
학술저널
저자정보
김태훈 (Department of Molecular Biology, Busan National University) 황미숙 (Seaweed Research Center, South Sea Fisheries Research Institute, National Fisheries Research and Development Institute) 송주동 (Department of Molecular Biology, Busan National University) 오민혁 (Department of Molecular Biology, Busan National University) 문용환 (Department of Molecular Biology, Busan National University) 정익교 (Department of Marine Science, Busan National University) 류태형 (Department of Biology, Busan National University) 이춘환 (Department of Molecular Biology, Busan National University)
저널정보
한국조류학회(藻類) Algae Algae 제21권 제2호
발행연도
2006.1
수록면
261 - 266 (6page)

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초록· 키워드

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The innate soluble polysaccharides and phenolic compounds of marine macroalgae are serious contaminants which interfere with experimental procedures such as restriction enzyme digestion, polymerase chain reaction (PCR) and other enzymatic reactions using extracted DNA samples. The viscous polysaccharides are co-precipitated with DNA samples by isopropanol or ethanol precipitation in conventional experiment. To overcome the problem, a method for the isolation of high molecular weight DNA from Porphyra tenera is developed with the application of diatomaceous earth column. The isolated DNAs by this method were about 50-100 kb in size and could be digested well with restriction enzymes. The nuclease activity seemed to be minimal, and high reproducibility in the arbitrary primed PCR for RAPD analyses was a distinctive feature. These results suggest that this method is very efficient in isolating nucleic acid from macroalgae including Porphyra.

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