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UV and high performance liquid chromatographic methods for the quantitative analysis of methyl 5-hydroxy-dinaphtho [1,2-2',3'] furan-7,12-dione-6-carboxylate(MHDDC) in urine and blood were developed. The correlation coefficients of the calibration curves of MHDDC in chloroform, methanol and dioxane solution were 0.999, 0.997 and 0.998, respectively. MHDDC was resolved within 15 min and had a detection limit of 2-5ng at S/N=3 by using a reversed-phase column with two solvents (MeOH, HAc).
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