지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Patterns of amylase secretion in mouse pancreatic fragments were studied over a period of time after the tissue was stimulated by acetyicholine and MNNG. MNNG is known to activate guanylate cyclase and thus increase the cGMP concentration in the pancreatic acinar cell. These amylase secretion patterns were studied to investigate the role of cGMP in reaction cascade during secretion response of the tissues stimulated by acetyicholine. Cellular response of amylase secretion in the pancreas by acetyicholine was divided into two phases. During the first phase, zymogen granules which had existed in the cells were secreted by the action of $Ca^2$+ and calmodulin immediately after secretagogue administration, this being known as the initial response. When the tissue was stimulated by acetylcholine in a $Ca^2$+-deficient medium or one containing trifluoperazine as a calmodulin antagonist, this initial response was reduced. In the second phase, newly formed zymogen granules were secreted as sustained response after protein synthesis was triggered by secretagogue. This response was provoked by an activation of protein kinase C. When either cycloheximide as a protein synthesis inhibitor or dibucaine as a protein kinase C inhibitor were added to the incubation medium, this sustained response was remarkablely depressed in the pancreatic fragments stimulated with acetylcholine. In the pancreatic acinar cell, phosphatidylinositol turnover plays an important role in the secretion response and hexachlorocyclohexane inhibits this phosphatidylinositol turnover. The pancreatic tissue treated with the hexachlorocyclohexane exhibited inhibition on both initial and sustained responses of amylase secretion by acetylcholine. MNNG also accelerated amylase secretion from the tissue gradually along incubation time. The 22 minutes fraction of the pancratic secretion after administration of both acetylcholine and MNNG showed higher amylase activity than the neighboring fractions. Guanylate cyclase potentiated the sustained response. Even if it is experimented with an indirect method, guanylate cyclase was found responsible for activation of the sustained response of a step prior to the action of protein kinase C. As conclusion, it was considered that amylase secretion in mouse pancreatic fragments stimulated by acetylcholine is a three phasic response.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Development of Secretion System of Anti-Tumor Protein using Type III Secretion System
한국미생물학회 학술대회논문집
2012 .05
-
Construction of Secretion Vectors Using the α-amylase Signal Sequence of Bacillus subtilis NA64
The Journal of Microbiology
1996 .03
-
Application of Yeast Secretion System for Large Production and Secretion Analysis of Human $\alpha$$_1$-Antitrypsin
한국동물학회 학술대회
1995 .01
-
NO and TNF-$\alpha$ Secretion on LPS-stimulated Murine Macrophage Are Inhibited by the Stress Response
한국동물학회 학술대회
1998 .01
-
대맥종자의 Amylase 생성에 미치는 Gibberellic Acid의 촉진효과와 Indole-3-acetic Acid의 억제효과의 해석
식물학회지
1977 .01
-
The Effect of Light on Baker’s Yeast Cell Growth and Protein Secretion
미생물학회지
1988 .03
-
Amylase 生成細菌에 關한 硏究 (第1報) : Amylase 生成細菌의 分離와 그 培養條件에 對하여
미생물학회지
1964 .10
-
Effects of pH Shock on the Secretion System in Streptomyces coelicolor A3(2)
Journal of Microbiology and Biotechnology
2008 .01
-
The stimulatory effect of IL-1β on the insulin secretion of rat pancreatic islet is not related with iNOS pathway
Experimental and Molecular Medicine
2002 .01
-
한국인의 성분비액에서의 Phosphoglucomutase-1(PGM$_1$)아형에 관한 연구
동물학회지
1988 .01
-
전분으로부터 α-Amylase에 의한 배당체의 합성
미생물학회지
1998 .09
-
Bacillus licheniformis의 내열성 α-amylase 및 maltogenic amylase 유전자의 분리와 그 효소 특성
한국미생물학회 학술대회논문집
1991 .04
-
Annexin I Stimulates Insulin Secretion through Regulation of Cytoskeleton and PKC Activity
Animal cells and systems
2009 .01
-
Secretion of Lignin Peroxidase H8 via the Flagellar Secretion Apparatus in Pseudomonas putida KT2440
한국미생물학회 학술대회논문집
2019 .04
-
Exploration and Exploitation of Protein Secretion Pathway in the World of Yeasts
한국미생물학회 학술대회논문집
2012 .05
-
Aspergillus nidulans에서 MNNG 선 처리시의 생존도와 돌연변이 유발에 대한 Adaptive response 및 Cell stage 따른 UV와 MNNG에 대한 치사율 조사
자연 과학 논문집 : 배재대학 첨단과학연구소
1997 .01
-
Characterization of Alpha-Amylase from Aspergillus niger Aggregate F Isolated from a Fermented Cassava Gatot Grown in Potato Peel Waste Medium
한국미생물·생명공학회지
2019 .01
-
사찰의 된장에서 분리된 Bacillus licheniformis YB-1234의 내열성 α-Amyalse
한국미생물·생명공학회지
2012 .01
-
대맥종자의 Amylase 생성에 미치는 Gibberellic Acid의 효과
식물학회지
1971 .01
-
Secretion Capacity Limitations of the Sec Pathway in Escherichia coli
Journal of Microbiology and Biotechnology
2004 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0