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논문 기본 정보

자료유형
학술저널
저자정보
Lee, Hyo-Shin (College of Agriculture, Kyungpook National University) Chung, Min-Sup (College of Agriculture, Kyungpook National University) Jo, Jin-Ki (College of Agriculture, Kyungpook National University)
저널정보
한국초지조사료학회 한국초지조사료학회지 한국초지조사료학회지 제18권 제1호
발행연도
1998.1
수록면
69 - 76 (8page)

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This study was conducted to construct of the transgenic plants wliich are resistant to oxidative stresses including ozone with B. mpestris cytosolic glutathione reductase cDNA using the binary vector system of Agrobacterium tumefaciens. The 1.8kb B. campestris cytosolic GR cDNA was subcloned into the unique Sma I site of the plant transformation vector pBKSI- I, downstream of the constitutive CaMV 35s promoter and upstream of the nos termination sequence, in place of the uidA (GUS) reporter gene. The resulting plant transformation vector, pBKS-GRI, was introduced into A. tumefaciens LBA4404 by two cycles of tkeze-thaw method. The B. nqus cotyledonary petioles were transformed by the Agrubaferium harboring pBKS-GRI. Transformed shoots were induced and selected on regeneration medium supplemented with kanarnycin. The shoot formation was increased remarkably by addition of Ag$NO_3$, in MS media. The transgenic plants were analyzed for the presence of the B. campestris GR gene by Southern blot analysis and it was confirmed that a foregin gene was stably integrated into the genomes of B. nqus plants.

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