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논문 기본 정보

자료유형
학술저널
저자정보
Park, Sang-Chan (Lab. of Cell Engineering & 3-D Structure, School of Life Sciences and Biotechnology, Korea University) Kang, Seok-Woo (Sericulture & Entomology, National Institute of Agricultural Science and Technology, Rural Development Administration) Han, Sung-Sik (Lab. of Cell Engineering & 3-D Structure, School of Life Sciences and Biotechnology, Korea University) Wago, Haruhisa (Lap. of Immunology, Department of Medical Technology, Junior college, Saitama Medical School)
저널정보
한국곤충학회 한국곤충학회지 한국곤충학회지 제33권 제1호
발행연도
2003.1
수록면
17 - 23 (7page)

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There is a pair of labial gland of hydropsychid caddisfly (Hydropsyche kozhantschikovi Martynov) larva. It is in ‘Z’ formation in the body and the total length is about 20mm. Hydropsychid caddishfly larvae that were raised under the lab conditions were able to form a nest-spining by connecting the small grains of sand provided. By repeatedly treating the extracted labial gland with methanol/D.W., the cell layer was removed. Accordingly, only the matrix within the labial gland that did not dissolve in water was obtained. The matrix inside the methanol/D.W. treated labial gland was dissolved with 5% acetic acid. Then the results of an acidic electrophoresis with a number of conditions indicated that 5% acetic acid/ 5M urea/ 8% PAGE was the most effective. Moreover, the result of 2-D PAGE on the labial gland of these hydropsychid caddisfly larva, the number of proteins in the labial gland including the cell layer was about 350 and the number of proteins in the labial gland treated with methanol/D.W. was about 80, showing a substantially small number of proteins.

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