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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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이 논문의 연구 히스토리 (2)
2003
Pharmacological Study of Intracellular Second Messengers that Affect Active Ion Transport in the Midgut of Tobacco Hornworm, Manduca sexta
Lee
,
Kyeong-Yeoll
한국곤충학회지
2003.01
학술저널
Pharmacological Study of Intracellular Second Messengers that Affect Active Ion Transport in the Midgut of Tobacco Hornworm, Manduca sexta
Lee
,
Kyeong-Yeoll
한국응용곤충학회 학술발표회
2003.01
학술대회자료
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Midgut active ion transport changes during the final larval stage of the tobacco hornworm. The short-circuit current ( $I_{sc}$ ) of the posterior midguts dissected from feeding fifth instars (day 2) is higher than that of midguts from wandering larvae (day 5). The $I_{sc}$ of midguts from day 2 larvae is inhibited by 1 mM cAMP and 0.1 mM cGMP, whereas the midguts from day 5 larvae are stimulated by cAMP but unaffected by cGMP. A similar pattern is observed if the midguts are exposed to the cyclic nucleotide derivative, 8-bromo-cGMP. Exposure to the calcium ionophore, A23187, or the endoplasmic calcium ATPase inhibitor, thapsigargin, slightly inhibited the $I_{sc}$ of day 2 larval midguts, but this inhibition was not significant. Pharmacological agents known to modulate the activities of protein kinase A, protein kinase C, or protein phosphatases did not change the Isco These results indicate that midgut active ion transport is modulated by cyclic nucleotides, but the manner of the response depends on the developmental status of the insect.
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Pharmacological Study of Intracellular Second Messengers that Affect Active Ion Transport in the Midgut of Tobacco Hornworm, Manduca sexta
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