지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Objective : The aim of this study was to evaluate the influence of different media on blastulation, mean cell number, percentage of inner cell mass (ICM) of total cells and ICM : trophectoderm (TE) ratio in mice. Materials and methods: A total 552 two cell embryos were retrieved from ICR female mice (4 weeks old) at 48 hr after hCG injection (mated just after hCG injection) and cultured in MEM (n=276) or TCM (n=276) supplemented with 20% hFF. The grading of blastocysts from zona-intact (ZiB) to -escape (hatching and hatched, ZeB) was performed at 72 hours after culture. Total, TE and ICM cell numbers were analyzed by differential staining of blastocyst. Statistical analysis was performed using t-test with SigmaPlot-2001. P-values < 0.05 were accepted as statistically significant. Results: The blastulation rate in MEM ($64.9{\pm}4.95%$) was significantly higher (p=0.0031) than that in TCM ($57.2{\pm}5.22%$). No differences were found in the number of ZiB and ZeB between MEM ($31.9{\pm}2.62$, $33.0{\pm}4.58%$), and TCM ($27.2{\pm}4.28$, $30.1{\pm}4.58%$). A total 314 blastocysts (MEM=166; TCM=148) were stained differentially. Mean cell number of blastocysts was significantly higher (p=0.0002) in TCM ($73.1{\pm}3.3$) than in MEM ($61.7{\pm}2.5$). Differential staining was successfully performed in 155 blastocysts (MEM=77; TCM=78). The percentage of ICM was significantly higher in MEM than in TCM ($20.9{\pm}1.3$ vs. $17.1{\pm}1.2%$, p=0.0281). The ICM : TE ratio was higher in TCM than in MEM (1 : $4.85{\pm}0.68$ vs. 1 : $3.78{\pm}0.78$, NS). Conclusion: These results show that MEM increase the blastocyst formation and percentage of ICM of total cells comparing with TCM in mice.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Effects of Media Volume on Blastocyst Formation, Cell Numbers and ICM Proportion in Mouse Two-cell Embryos
대한불임학회지
2004 .01
-
Effect of Epidermal Growth Factor on In Vitro Maturation in Pig Immature Oocytes;IV. Effect of Epidermal Growth Factor on In Vitro Development
대한불임학회잡지
1996 .01
-
ICM - Trophectoderm Cell Numbers of Mouse IVF/IVC Blastocysts
대한불임학회잡지
1996 .01
-
The effect of the site of laser zona opening on the complete hatching of mouse blastocysts and their cell numbers
Clinical and experimental reproductive medicine : CERM
2016 .01
-
Human Blastocysts;The Correlation Between Embryo Microscopical Assessments and Their Cell Number
대한불임학회잡지
1996 .01
-
지연형 조영제 과민반응의 임상 양상
Allergy Asthma & Respiratory Diseases
2014 .01
-
생쥐 배의 배반포 형성과 세포수에 미치는Glucose의 영향
Obstetrics & Gynecology Science
2004 .01
-
In Vitro Developments of Porcine Embryos Produced by In Vitro Fertilization and Parthenogenetic Activation
대한불임학회잡지
1997 .01
-
동결-융해된 인간 배반포기 배 유래의 배아 간(幹) 세포 배양
대한불임학회지
2001 .01
-
체외수정 생쥐 배아에서의 배아 줄기세포 확립
대한불임학회지
2002 .01
-
A retrospective study of single frozen-thawed blastocyst transfer
Clinical and experimental reproductive medicine : CERM
2016 .01
-
The Effects of Glucose on Blastulation and Cell Counts of Blastocysts in Mice
대한생식의학회 학술대회
2003 .01
-
요오드화 조영제에 의한 고정약진 1예
Allergy Asthma & Respiratory Diseases
2015 .01
-
Brief Introduction of Traditional Chinese Medicine(TCM) and Possible Measures to Protect the Endangered Species of TCM
대한한의학회지
1997 .10
-
Reliability and Concurrent Validity of Korean Version of the Trunk Control Measurement Scale (K-TCMS) for Children with Cerebral Palsy
대한물리치료학회지(JKPT)
2017 .01
-
인간태아 섬유아세포와 생쥐배아 섬유아세포를 기저세포로 활용한 인간 배아줄기세포의 확립
대한불임학회지
2005 .01
-
생쥐 내세포괴의 분리방법과 지지세포의 종류와 Mitomycin C 처리 시간이 내세포괴 Colony 형성률에 미치는 영향
대한불임학회지
2006 .01
-
Effect of cell-penetrating peptide-conjugated estrogen-related receptor ${\beta}$ on the development of mouse embryos cultured in vitro
Clinical and experimental reproductive medicine : CERM
2014 .01
-
Expression of Epidermal Growth Factor-Receptor (EGF-R) on the Human Embryos Developed from IVF Multi-Pronucleated zygotes and Inner cell mass Cells (ICMs)
대한생식의학회 학술대회
1997 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0